HSP22 Antibody | StressMarq Biosciences SMC-187D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP22 Antibody

catalog :

SMC-187D

quantity :

100 µg

price :

300.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C12-H11

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-187D

Product Name :

HSP22 Antibody

Description :

Mouse Anti-Human HSP22 Monoclonal IgG1 Kappa

Target :

HSP22

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Neuroscience Neurodegeneration

Alternative Name(s) :

Alpha crystallin C chain Antibody, CMT2L Antibody, CRYAC Antibody, DHMN2 Antibody, H11 Antibody, Heat shock 22kDa protein 8 Antibody, HMN2 Antibody, HSB8 Antibody, HSPB8 Antibody, HSP22 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

3C12-H11

Immunogen :

His-tagged human recombinant HSP22

Immunogen Species :

Human

Accession Number :

NP_055180.1

Swiss-Prot :

Q9UJY1

Applications :

WB IHC ICC/IF ELISA

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:2000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~22kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-187D, RRID: AB_10640879

Certificate of Analysis :

1 µg/ml of SMC-187 was sufficient for detection of HSP22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6).

References :

1.Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6. 2. Benndorf R., et al. (2001) J Biol Chem 276: 26753-26761. 3.Sun X., et al. (2004) J Biol Chem 279: 2394-2402. 4.Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652. 5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-187_Hsp22_Antibody_3C12-H11_IHC_Mouse_backskin_1.png SMC-187_Hsp22_Antibody_3C12-H11_WB_Rat_Cell-lysates_1.png

more info or order :

StressMarq Biosciences product webpage