This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
StressMarq Biosciences
product type :
antibody
product name :
HIF2 alpha Antibody
catalog :
SMC-185D
quantity :
100 µg
price :
268.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ep190b
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000
Lin K, Lien J, Chung C, Kuo S, Huang T. Bp5250 inhibits vascular endothelial growth factor-induced angiogenesis and HIF-1? expression on endothelial cells. Naunyn Schmiedebergs Arch Pharmacol. 2012;385:39-49 pubmed publisher
product information
Catalog No :
SMC-185D
Product Name :
HIF2 alpha Antibody
Description :
Mouse Anti-Human HIF2 alpha Monoclonal IgG1
Target :
HIF 2 alpha
Conjugate :
Unconjugated
2021 List Price :
268.00 USD
Currency :
USD
Research Area(s) :
Cancer Oxidative Stress Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
ECYT4 Antibody, EPAS1 Antibody, HIF2alpha Antibody, HIF1 Alpha like factor Antibody, HLF Antibody, Hypoxia inducible factor 2 alpha Antibody, MOP2 Antibody, PASD2 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
EP190B
Immunogen :
GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein
Immunogen Species :
Human
Accession Number :
NP_001421.4
Swiss-Prot :
Q99814
Applications :
WB IHC ICC/IF ELISA AM
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms
Species Reactivity Full Name :
Human Mouse
Antibody Dilution :
WB (1:500), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~100kDa. Specific for HIF2 Alpha.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-185D, RRID: AB_10643260
Certificate of Analysis :
1 µg/ml of SMC-185 was sufficient for detection of HIF2α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus
Scientific Background :
Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia (1). HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. It is overexpressed in many cancerous tissues, but its exact role in tumourprogression remains to be clarified (2). Studies suggest that in the case of non-small cell lung cancer, HIF2-Alpha actually is a promoter of tumor growth and progression in a solid tumor (1). Other data suggests that HIF2-Alpha is an important regulator of innate immunity, and therefore may be useful in the therapeutic target for treating inflammatory disorders and cancer (3).
References :
1. Kim W.Y., et al. (2009) J Clin Invest. 119(8): 2160-2170. 2. Favier J., Lapointe S., Maliba R. and Sirois M.G. (2007) BMC Cancer. 7:139. 3. Imtiyaz H.Z., et al. (2010) J Clin Invest. 120(8): 2699-2714.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-185_HIF2-alpha_Antibody_EP190b_ICC-IF_Human_HeLa-Cells_20x_Composite.png
SMC-185_HIF2-alpha_Antibody_EP190b_IHC_
Mouse_backskin_1.png
SMC-185_HIF2-alpha_Antibody_EP190b_ICC-IF_Human_HeLa-Cells_100x_Composite.png SMC-185_HIF2-alpha_Antibody_EP190b_WB_Human_HeLa-cell-lysates_1.png
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.