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company name :
StressMarq Biosciences
product type :
antibody
product name :
HIF1 alpha Antibody
catalog :
SMC-184D
quantity :
100 µg
price :
268.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ESEE122
reactivity :
human, mouse, rat, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
Surazynski A, Miltyk W, Prokop I, Palka J. The effect of estrogen on prolidase-dependent regulation of HIF-1? expression in breast cancer cells. Mol Cell Biochem. 2013;379:29-36 pubmed publisher
product information
Catalog No :
SMC-184D
Product Name :
HIF1 alpha Antibody
Description :
Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1
Target :
HIF 1 alpha
Conjugate :
Unconjugated
2021 List Price :
268.00 USD
Currency :
USD
Research Area(s) :
Cancer Oxidative Stress Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
ARNT interacting protein Antibody, HIF1A Antibody, Hypoxia inducible factor 1 alpha Antibody, MOP1 Antibody, PASD8 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
ESEE122
Immunogen :
Recombinant fragment corresponding to amino acids 329-530
Immunogen Species :
Mouse
Accession Number :
NP_034561.2
Swiss-Prot :
Q61221
Applications :
WB IHC ICC/IF ELISA
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt Bv
Species Reactivity Full Name :
Human Mouse Rat Bovine
Antibody Dilution :
WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~116kDa. Specific for HIF1Alpha.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-184D, RRID: AB_10640132
Certificate of Analysis :
1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus
Scientific Background :
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
References :
1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4. 3. Jaakkola P., et al. (2001) Science 292: 468-72. 4. Maxwell P.H., et al. (1999) Nature 399: 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9. 7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-184_HIF1-alpha_Antibody_ESEE122_WB_Human_HeLa-cell-lysates_1.png
SMC-184_HIF1-alpha_Antibody_ESEE122_IHC
_Mouse_backskin_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.