This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
StressMarq Biosciences
product type :
antibody
product name :
FIH Antibody
catalog :
SMC-182D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FIH 162c
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry
product information
Catalog No :
SMC-182D
Product Name :
FIH Antibody
Description :
Mouse Anti-Human FIH Monoclonal IgG1
Target :
FIH
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Cancer Oxidative Stress Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
Factor inhibiting HIF1 (hypoxia-inducible factor) Antibody, DKFZp762F1811 Antibody, Factor inhibiting HIF-1 Antibody, Factor inhibiting HIF1 Antibody, FIH 1 Antibody, FIH-1 Antibody, FIH1 Antibody, FLJ20615 Antibody, FLJ22027 Antibody, HIF1AN Antibody, HIF1N_HUMAN Antibody, Hypoxia inducible factor 1 alpha inhibitor Antibody, Hypoxia inducible factor 1 alpha subunit inhibitor Antibody, Hypoxia inducible factor asparagine hydroxylase Antibody, Hypoxia-inducible factor 1-alpha inhibitor Antibody, Hypoxia-inducible factor asparagine hydroxylase Antibody, Peptide aspartate beta dioxygenase Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
FIH 162c
Immunogen :
Full length human FIH expressed in E.coli BL21 (DE3) cells
Immunogen Species :
Human
Accession Number :
NP_060372.2
Swiss-Prot :
Q9NWT6
Applications :
WB IHC ICC/IF
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms
Species Reactivity Full Name :
Human Mouse
Antibody Dilution :
WB (1:100), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~45kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-182D, RRID: AB_10644174
Certificate of Analysis :
1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus
Scientific Background :
FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional co-activators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia (1-3). Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell carcinoma (4).
References :
1. Stolze I.P., et al. (2004) J Bio Chem. 42719-42725. 2. Soilleux E.J., et al. (2005) Histopathology 47:602-610. 3. Moon H., Han S., Park H., Choe J. (2010) Mol Cells. 29(5): 471-474. 4. Kroeze S.G., et al. (2010) Eur J Cancer. Epub.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-182_FIH_Antibody_fih162C_ICC-IF_Human_HeLa-Cells_20x_Composite.png SMC-182_FIH_Antibody_fih162c_WB_Human_Cell-lysates_1.png
SMC-182_FIH_Antibody_fih162C_IHC_Mouse_
backskin_1.png
SMC-182_FIH_Antibody_fih162C_ICC-IF_Human_HeLa-Cells_100x_Composite.png
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.