Rhodopsin Antibody | StressMarq Biosciences SMC-177D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Rhodopsin Antibody

catalog :

SMC-177D

quantity :

100 µg

price :

300.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D4

reactivity :

human, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:1000 western blot; human; 1:5000	Molday L, Molday R. 1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins. Methods Mol Biol. 2014;1177:1-15 pubmed publisher
immunocytochemistry; human	Cheng C, Djajadi H, Molday R. Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy. Methods Mol Biol. 2013;935:185-99 pubmed publisher

product information

Catalog No :

SMC-177D

Product Name :

Rhodopsin Antibody

Description :

Mouse Anti-Bovine Rhodopsin Monoclonal IgG1

Target :

Rhodopsin

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Neurotransmitter Receptors Rhodopsins Cell Signaling

Alternative Name(s) :

OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

1D4

Immunogen :

Bovine Rhodopsin

Immunogen Species :

Bovine

Accession Number :

NP_001014890.1

Swiss-Prot :

P02699

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Species Reactivity Full Name :

Vertebrate

Antibody Dilution :

WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-177D, RRID: AB_10600312

Certificate of Analysis :

1 µg/ml of SMC-177 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Membrane

Scientific Background :

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

References :

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-177_Rhodopsin_Antibody_1D4_WB_Human_Cell-lysates_1.png
SMC-177_Rhodopsin_Antibody_1D4_IHC_Mous
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more info or order :

StressMarq Biosciences product webpage