product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Rhodopsin Antibody
catalog :
SMC-177
quantity :
25 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D4
reactivity :
human, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 3
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:1000
  • western blot; human; 1:5000
Molday L, Molday R. 1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins. Methods Mol Biol. 2014;1177:1-15 pubmed publisher
  • immunocytochemistry; human
Cheng C, Djajadi H, Molday R. Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy. Methods Mol Biol. 2013;935:185-99 pubmed publisher
Makowka P, Bruegmann T, Dusend V, Malan D, Beiert T, Hesse M, et al. Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision. Nat Commun. 2019;10:1281 pubmed publisher
image
image 1 :
StressMarq Biosciences SMC-177 image 1
Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (SMC-177). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (SMC-177) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
product information
Catalog No :
SMC-177
Product Name :
Rhodopsin Antibody
Description :
Mouse Anti-Bovine Rhodopsin Monoclonal IgG1
Target :
Rhodopsin
Research Area(s) :
Neuroscience Neurotransmitter Receptors Rhodopsins Cell Signaling
Alternative Name(s) :
OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
1D4
Immunogen :
Bovine Rhodopsin
Accession Number :
NP_001014890.1
Swiss-Prot :
P02699
Applications :
WB IHC ICC/IF IP ELISA
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Medium :
Vertebrate
Antibody Dilution :
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Specificity :
Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Cellular Localization :
Membrane
Size 1 :
25 µg
Size 2 :
100 µg
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.