Rhodopsin Antibody | StressMarq Biosciences SMC-176D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Rhodopsin Antibody

catalog :

SMC-176D

quantity :

100 µg

price :

300.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D2

reactivity :

mouse, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse	Cheng C, Djajadi H, Molday R. Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy. Methods Mol Biol. 2013;935:185-99 pubmed publisher
	Tanaka G, Parker A, Hasegawa Y, Siveter D, Yamamoto R, Miyashita K, et al. Mineralized rods and cones suggest colour vision in a 300 Myr-old fossil fish. Nat Commun. 2014;5:5920 pubmed publisher

image

image 1 :

Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (SMC-176). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (SMC-176) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS – inner segment; ONL – outer nuclear layer; OPL – outer plexiform layer; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.

product information

Catalog No :

SMC-176D

Product Name :

Rhodopsin Antibody

Description :

Mouse Anti-Bovine Rhodopsin Monoclonal IgG1

Target :

Rhodopsin

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Neurotransmitter Receptors Rhodopsins Cell Signaling

Alternative Name(s) :

OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

4D2

Immunogen :

Bovine Rhodopsin

Immunogen Species :

Bovine

Accession Number :

NP_001014890.1

Swiss-Prot :

P02699

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Mm Fs Av Am Shk

Species Reactivity Full Name :

Mammals Fish Anchovies Avian Amphibians Shark Extinct Spiny Shark (Acanthodes bridgei)

Antibody Dilution :

WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-176D, RRID: AB_10599810

Certificate of Analysis :

1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Membrane

Scientific Background :

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

References :

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-176_Rhodopsin_Antibody_4D2_WB_Human_A549-cells_1.png
SMC-176_Rhodopsin_Antibody_4D2_IHC_Mous
e_retina_1.png

more info or order :

StressMarq Biosciences product webpage