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company name :
StressMarq Biosciences
product type :
antibody
product name :
HDEL Antibody
catalog :
SMC-175D
quantity :
100 µg
price :
310.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2E7
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-175D
Product Name :
HDEL Antibody
Description :
Mouse Anti-Yeast HDEL Monoclonal IgG2b
Target :
HDEL
Conjugate :
Unconjugated
2021 List Price :
310.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking
Alternative Name(s) :
H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
2E7
Immunogen :
Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Immunogen Species :
Yeast
Applications :
WB ICC/IF
Host Species :
Mouse
Isotype :
IgG2b
Species Reactivity Abbreviation :
Dr Ys Pl
Species Reactivity Full Name :
Fruit Fly (Drosophila melanogaster) Yeast Yeast (Saccharomyces cerevisiae) Plant Barnyard Grass (Echinochloa) Beet (Beta vulgaris) Cotton (Gossypium) Mung Bean (Vigna radiata) Grass (Sorghum) Wheat (Triticum spp.)
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~78kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-175D, RRID: AB_10556216
Certificate of Analysis :
1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Endoplasmic Reticulum
Scientific Background :
HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
References :
1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.