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company name :
StressMarq Biosciences
product type :
antibody
product name :
AHA1 Antibody
catalog :
SMC-173D
quantity :
100 µg
price :
314.00 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
25F2.D10
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-173D
Product Name :
AHA1 Antibody
Description :
Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa
Target :
AHA1
Conjugate :
Unconjugated
2021 List Price :
314.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins
Alternative Name(s) :
Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
25F2.D10
Immunogen :
Mouse Aha1
Immunogen Species :
Mouse
Accession Number :
NP_666148.1
Swiss-Prot :
Q8BK64
Applications :
WB IHC ICC/IF IP ELISA
Host Species :
Rat
Isotype :
IgG2a Kappa
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP ( 1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
Concentration :
1.59 mg/ml
Specificity :
Detects ~38kDa. Can run up to 45kDa on SDS Page.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-173D, RRID: AB_2224090
Certificate of Analysis :
1 µg/ml of SMC-173 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Endoplasmic Reticulum
Scientific Background :
Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
References :
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-173_Aha1_Antibody_25F2.D10_ICC-IF_Human_HeLa-cells_1.png SMC-173_Aha1_Antibody_25F2.D10_IP_Human_HeLa-cells_1.png SMC-173_Aha1_Antibody_25F2.D10_IHC_Mouse_backskin_2.png SMC-173_Aha1_Antibody_25F2.D10_WB_Human_Cell-lysates_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.