ERp57 (GRP58) Antibody | StressMarq Biosciences SMC-168B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

ERp57 (GRP58) Antibody

catalog :

SMC-168B

quantity :

200 µg

price :

314.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Map.ERp57

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human	Lynes E, Raturi A, Shenkman M, Ortiz Sandoval C, Yap M, Wu J, et al. Palmitoylation is the switch that assigns calnexin to quality control or ER Ca2+ signaling. J Cell Sci. 2013;126:3893-903 pubmed publisher
	Ortiz Sandoval C, Hughes S, Dacks J, Simmen T. Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins. Cell Logist. 2014;4:e986399 pubmed

product information

Catalog No :

SMC-168B

Product Name :

ERp57 (GRP58) Antibody

Description :

Mouse Anti-Human ERp57 (GRP58) Monoclonal IgG1

Target :

ERp57

Conjugate :

Unconjugated

2021 List Price :

314.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Protein Trafficking ER Proteins

Alternative Name(s) :

ERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Grp58 Antibody, P58 Antibody, PDIA3 Antibody, PI PLC Antibody, 58 kDa glucose regulated protein antibody, 58 kDa glucose-regulated protein antibody, 58 kDa microsomal protein antibody, Disulfide isomerase ER 60 antibody, Disulfide isomerase ER-60 antibody, Endoplasmic reticulum resident protein 57 antibody, Endoplasmic reticulum resident protein 60 antibody, ER p57 antibody, ER protein 57 antibody, ER protein 60 antibody, ERp 57 antibody, ERp57 antibody, Glucose Regulated Protein 58 Kd antibody, GRP 57 antibody, GRP 58 antibody, GRP57 antibody, HsT17083 antibody, p58 antibody, PDIA 3 antibody, PDIA3 antibody, PDIA3_HUMAN antibody, Phospholipase C alpha antibody, PI PLC antibody, Protein disulfide isomerase A3 antibody, Protein disulfide isomerase family A member 3 antibody, Protein disulfide-isomerase A3 antibody

Size :

200 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

Map.ERp57

Immunogen :

Human recombinant ERp57 (Grp58)

Immunogen Species :

Human

Accession Number :

NP_005304.3

Swiss-Prot :

P30101

Applications :

WB IHC ICC/IF IP

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt Bv Dg GP Hm Mk Pg Rb

Species Reactivity Full Name :

Human Mouse Rat Bovine Dog Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit

Antibody Dilution :

WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~57kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-168B, RRID: AB_2163133

Certificate of Analysis :

0.5 µg/ml of SMC-168 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Endoplasmic Reticulum Endoplasmic Reticulum Lumen Melanosome

Scientific Background :

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

References :

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-168_Erp57-Grp58_Antibody_Map.ERP57_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SMC-168_Erp57_Antibody_Map.ERP57_IHC_Mouse_backskin_2.png SMC-168_Erp57_Antibody_Map.ERP57_WB_Human_cell-lysates_1.png SMC-168_Erp57_Antibody_Map.ERP57_ICC-IF_Human_HaCaT-cells_1.png

more info or order :

StressMarq Biosciences product webpage