SOD (EC) Antibody | StressMarq Biosciences SMC-167D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

SOD (EC) Antibody

catalog :

SMC-167D

quantity :

100 µg

price :

294.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4GG11G6

reactivity :

, mice , human, mouse, rat, pigs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; pigs ; 1:1000	Konior A, Klemenska E, Brudek M, Podolecka E, Czarnowska E, Beresewicz A. Seasonal superoxide overproduction and endothelial activation in guinea-pig heart; seasonal oxidative stress in rats and humans. J Mol Cell Cardiol. 2011;50:686-94 pubmed publisher
immunohistochemistry; human; 1:2000 immunohistochemistry; mice ; 1:2000	Tollefson A, Oberley Deegan R, Butterfield K, Nicks M, Weaver M, Remigio L, et al. Endogenous enzymes (NOX and ECSOD) regulate smoke-induced oxidative stress. Free Radic Biol Med. 2010;49:1937-46 pubmed publisher

product information

Catalog No :

SMC-167D

Product Name :

SOD (EC) Antibody

Description :

Mouse Anti-Human SOD (EC) Monoclonal IgG1 Kappa

Target :

SOD3

Conjugate :

Unconjugated

2021 List Price :

294.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Cancer Cancer Metabolism Response to Hypoxia Cell Signaling Metabolism Metabolism processes Hypoxia Cell Signaling Metabolism Redox Metabolism

Alternative Name(s) :

EC SOD antibody, EC-SOD antibody, Extracellular superoxide dismutase [Cu Zn] antibody, Extracellular superoxide dismutase [Cu-Zn] antibody, Extracellular superoxide dismutase antibody, Extracellular superoxide dismutase precursor antibody, MGC20077 antibody, SOD 3 antibody, SOD3 antibody, SODE_HUMAN antibody, Superoxide dismutase 3 extracellular antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

4GG11G6

Immunogen :

Human extracellular SOD purified from aortas

Immunogen Species :

Human

Accession Number :

NP_003093.2

Swiss-Prot :

P08294

Applications :

WB IHC ICC/IF ELISA

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms Rt GP

Species Reactivity Full Name :

Human Mouse Rat Guinea Pig (Cavia porcellus)

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects extracellular SOD ~35kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-167D, RRID: AB_2191964

Certificate of Analysis :

1 µg/ml of SMC-167 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Extracellular Space

Scientific Background :

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

References :

1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O'Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-167_SOD3_Antibody_4GG11G6_WB_Human_
cartilage-lysates_1.png
SMC-167_SOD3_Antibody_4GG11G6_ICC-IF_Human_cartilage_1.png

more info or order :

StressMarq Biosciences product webpage