HSP40, YDJ1 Antibody | StressMarq Biosciences SMC-166D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP40, YDJ1 Antibody

catalog :

SMC-166D

quantity :

100 µg

price :

294.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A7.H6

reactivity :

Saccharomyces cerevisiae S288C, human

application :

western blot, ELISA, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6b	Sluder I, Nitika -, Knighton L, Truman A. The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity. PLoS Genet. 2018;14:e1007462 pubmed publisher

image

image 1 :

Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6 (SMC-166). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-166) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

product information

Catalog No :

SMC-166D

Product Name :

HSP40, YDJ1 Antibody

Description :

Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa

Target :

HSP40

Conjugate :

Unconjugated

2021 List Price :

294.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins

Alternative Name(s) :

DnaJ (HSP40) homolog subfmaily B member 1 antibody, DNAJ 1 antibody, DNAJ B1 antibody, DnaJ homolog subfamily B member 1 antibody, DnaJ protein homolog 1 antibody, DNAJ1 antibody, DNAJB 1 antibody, DNAJB1 antibody, DNAJB1 protein antibody, DNJB1_HUMAN antibody, HDJ 1 antibody, HDJ-1 antibody, HDJ1 antibody, Heat shock 40 kDa protein 1 antibody, Heat shock 40kD protein 1 antibody, Heat shock protein 40 antibody, HSP 40 antibody, HSP40 antibody, HSPF 1 antibody, HSPF1 antibody, Human DnaJ protein 1 antibody, Radial spoke 16 homolog B antibody, RSPH16B antibody, Sis1 antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

2A7.H6

Immunogen :

Full length protein HSP40 (YDJ1)

Immunogen Species :

Yeast

Accession Number :

NP_014335.1

Swiss-Prot :

P25491

Applications :

WB IP ELISA

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Species Reactivity Full Name :

Yeast

Antibody Dilution :

WB (1:2000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~40kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-166D, RRID: AB_2094596

Certificate of Analysis :

0.5 µg/ml of SMC-166 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

References :

1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91. 4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890. 7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-166_Hsp40_Antibody_2A7-H6_WB_Yeast_recombinant-cell-lysate_1.png

more info or order :

StressMarq Biosciences product webpage