HSP27 Antibody | StressMarq Biosciences SMC-161 product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP27 Antibody

catalog :

SMC-161

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D12-A12

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:1500; loading ...; fig 15 western blot; human; 1:1500; loading ...; fig 13a	Sugiyama S, Yoshino Y, Kuriyama S, Inoue M, Komine K, Otsuka K, et al. A Curcumin Analog, GO-Y078, Effectively Inhibits Angiogenesis through Actin Disorganization. Anticancer Agents Med Chem. 2016;16:633-47 pubmed
immunohistochemistry; human	Kötter S, Unger A, Hamdani N, Lang P, Vorgerd M, Nagel Steger L, et al. Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. J Cell Biol. 2014;204:187-202 pubmed publisher
	Gonçalves C, Sharon I, Schmeing T, Ramos C, Young J. The chaperone HSPB1 prepares protein aggregates for resolubilization by HSP70. Sci Rep. 2021;11:17139 pubmed publisher
	Mymrikov E, Riedl M, Peters C, Weinkauf S, Haslbeck M, Buchner J. Regulation of small heat-shock proteins by hetero-oligomer formation. J Biol Chem. 2020;295:158-169 pubmed publisher
	Wu D, Vonk J, Salles F, Vonk D, Haslbeck M, Melki R, et al. The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation-suppressing activity. J Biol Chem. 2019;294:9985-9994 pubmed publisher
	Meister Broekema M, Freilich R, Jagadeesan C, Rauch J, Bengoechea R, Motley W, et al. Myopathy associated BAG3 mutations lead to protein aggregation by stalling Hsp70 networks. Nat Commun. 2018;9:5342 pubmed publisher
	Winter L, Unger A, Berwanger C, Sporrer M, Turk M, Chevessier F, et al. Imbalances in protein homeostasis caused by mutant desmin. Neuropathol Appl Neurobiol. 2018;: pubmed publisher
	Pereira M, Tomé D, Domingues A, Varanda A, Paulo C, Santos M, et al. A Fluorescence-Based Sensor Assay that Monitors General Protein Aggregation in Human Cells. Biotechnol J. 2018;13:e1700676 pubmed publisher
	Unger A, Beckendorf L, Böhme P, Kley R, von Frieling Salewsky M, Lochmuller H, et al. Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere function. Acta Neuropathol Commun. 2017;5:72 pubmed publisher
	Lee S, Dempsey Hibbert N, Vimalachandran D, Wardle T, Sutton P, Williams J. Re-examining HSPC1 inhibitors. Cell Stress Chaperones. 2017;22:293-306 pubmed publisher
	Mymrikov E, Daake M, Richter B, Haslbeck M, Buchner J. The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins. J Biol Chem. 2017;292:672-684 pubmed publisher

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 5D12-A3 (SMC-161). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (SMC-161) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp27 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

product information

Catalog No :

SMC-161

Product Name :

HSP27 Antibody

Description :

Mouse Anti-Human HSP27 Monoclonal IgG2b Kappa

Target :

HSP27

Research Area(s) :

Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Cytoskeleton Microfilaments Actin Actin Assembly Cell Signaling Cytoskeleton Microtubules Cancer Tumor Biomarkers Cardiovascular System Atherosclerosis Cardiovascular System Heart Contractility Cancer Heat Shock

Alternative Name(s) :

28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

5D12-A12

Immunogen :

Full length human HSP27

Accession Number :

NP_001531.1

Swiss-Prot :

P04792

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Mouse

Isotype :

IgG2b Kappa

Species Reactivity Medium :

Human

Antibody Dilution :

WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Specificity :

Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.

Cellular Localization :

Cytoplasm Nucleus Cytoskeleton Spindle

Size 1 :

50 µg

Size 2 :

200 µg

more info or order :

StressMarq Biosciences product webpage