p23 Antibody | StressMarq Biosciences SMC-156D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p23 Antibody

catalog :

SMC-156D

quantity :

100 µg

price :

247.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JJ6

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; 1:250 western blot; human	Shimamura T, Perera S, Foley K, Sang J, Rodig S, Inoue T, et al. Ganetespib (STA-9090), a nongeldanamycin HSP90 inhibitor, has potent antitumor activity in in vitro and in vivo models of non-small cell lung cancer. Clin Cancer Res. 2012;18:4973-85 pubmed publisher

product information

Catalog No :

SMC-156D

Product Name :

p23 Antibody

Description :

Mouse Anti-Human p23 Monoclonal IgG1

Target :

p23

Conjugate :

Unconjugated

2021 List Price :

247.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Epigenetics and Nuclear Signaling Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Metabolism Lipid and Lipoprotein Metabolism

Alternative Name(s) :

co chaperone p23 Antibody, PTGES3 Antibody, TEBP Antibody, telomerase binding protein p23 Antibody, unactive progesterone receptor 23kDa Antibody, HSP90 co-chaperone Antibody, Progesterone receptor complex p23 Antibody, Cytosolic prostaglandin E2 synthase Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

JJ6

Immunogen :

Recombinant human full length p23 protein

Immunogen Species :

Human

Accession Number :

NP_006592.3

Swiss-Prot :

Q15185

Applications :

WB IHC ICC/IF IP ELISA AM

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rb Ck GP Ys

Species Reactivity Full Name :

Human Mouse Rabbit Chicken Guinea Pig (Cavia porcellus) Yeast Yeast (Saccharomyces cerevisiae)

Antibody Dilution :

WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~23kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-156D, RRID: AB_1055466

Certificate of Analysis :

0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm

Scientific Background :

p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.

References :

1. Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-156_p23_Antibody_JJ6_WB_Human_Cell-lysates_1.png SMC-156_p23_Antibody_JJ6_ICC-IF_Human_HaCaT-cells_1.png
SMC-156_p23_Antibody_JJ6_IHC_Mouse_back
skin_2.png
SMC-156_p23_Antibody_JJ6_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png

more info or order :

StressMarq Biosciences product webpage