DNA/RNA Damage Antibody | StressMarq Biosciences SMC-155D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

DNA/RNA Damage Antibody

catalog :

SMC-155D

quantity :

100 µg

price :

278.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

15A3

reactivity :

human

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

StressMarq Biosciences product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	Aboud O, Mrak R, Boop F, Griffin W. Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype. Acta Neuropathol Commun. 2013;1:41 pubmed publisher
	Wu P, Scarlata E, O Flaherty C. Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa. Antioxidants (Basel). 2020;9: pubmed publisher
	Angeloni C, Teti G, Barbalace M, Malaguti M, Falconi M, Hrelia S. 17β-Estradiol enhances sulforaphane cardioprotection against oxidative stress. J Nutr Biochem. 2017;42:26-36 pubmed publisher
	Liu Y, O Flaherty C. In vivo oxidative stress alters thiol redox status of peroxiredoxin 1 and 6 and impairs rat sperm quality. Asian J Androl. 2017;19:73-79 pubmed publisher
	Lood C, Blanco L, Purmalek M, Carmona Rivera C, De Ravin S, Smith C, et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nat Med. 2016;22:146-53 pubmed publisher
	Angeloni C, Malaguti M, Rizzo B, Barbalace M, Fabbri D, Hrelia S. Neuroprotective effect of sulforaphane against methylglyoxal cytotoxicity. Chem Res Toxicol. 2015;28:1234-45 pubmed publisher
	Ozkosem B, Feinstein S, Fisher A, O Flaherty C. Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice. Redox Biol. 2015;5:15-23 pubmed publisher
	Zawada W, Mrak R, Biedermann J, Palmer Q, Gentleman S, Aboud O, et al. Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related nucleic acid oxidation and caspase-3 activation. Acta Neuropathol Commun. 2015;3:9 pubmed publisher
	Brennan L, Haukedal J, Earle J, Keddie B, Harris H. Disruption of redox homeostasis leads to oxidative DNA damage in spermatocytes of Wolbachia-infected Drosophila simulans. Insect Mol Biol. 2012;21:510-20 pubmed publisher

product information

Catalog No :

SMC-155D

Product Name :

DNA/RNA Damage Antibody

Description :

Mouse Anti- DNA/RNA Damage Monoclonal IgG2b

Target :

DNA Damage

Conjugate :

Unconjugated

2021 List Price :

278.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Cell Signaling Post-translational Modifications Oxidation Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Damage and Repair

Alternative Name(s) :

8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, 8OHG Antibody, 80G Antibody, 8 hydroxyguanine Antibody, 8 hydroxy 2' deoxyguanosine Antibody, 8 hydroxyguanosine Antibody, 8 OHG Antibody, 8-OHG Antibody, 8OHdG Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Trademark :

MOLECULAR SIGNATURE®

Clone Number :

15A3

Immunogen :

8-hydroxy-guanosine-BSA and –casein conjugates

Applications :

IHC ICC/IF ELISA DB IP FCM FA

Host Species :

Mouse

Isotype :

IgG2b

Species Reactivity Abbreviation :

ALL

Species Reactivity Full Name :

Species Independent

Antibody Dilution :

IHC (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-155D, RRID: AB_889490

Scientific Background :

DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).

References :

1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241. 2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup Environ Health. 80(1): 1-15. 3. Malins D.C. and Haimanot R. (1991) Cancer Res. 51(19): 5430-5432. 4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11): 2281-2283. 5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest Ophthalmol Vis Sci 47(4): 1594-9. 6. Bowers R. et al. (2004) Am J Respir Crit Care Med. 169(6): 764-9. 7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000) Faseb J. 14(7): 955-67.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-155_DNA-Damage_Antibody_15A3_IHC_Rat_ischemic-fresh-brain-tissue_1.png
SMC-155_DNA-Damage_Antibody_15A3_IHC_Mo
use_backskin_1.png
SMC-155_DNA-Damage_Antibody_15A3_IHC_Mouse_Retinal-Injury-Model_1.png SMC-155_DNA-Damage_Antibody_15A3_IHC_Mouse_inflamed-colon_40x_1.png

more info or order :

StressMarq Biosciences product webpage