Nitrotyrosine Antibody | StressMarq Biosciences SMC-154D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Nitrotyrosine Antibody

catalog :

SMC-154D

quantity :

100 µg

price :

312.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

39B6

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

StressMarq Biosciences product webpage

citations: 9

Reference
Hovestad Bijl L, van Ameijde J, Pijnenburg D, Hilhorst R, Liskamp R, Ruijtenbeek R. Peptide Microarrays for Real-Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples. Methods Mol Biol. 2016;1447:67-78 pubmed publisher
Ampem G, Azegrouz H, Bacsadi Ã, Balogh L, Schmidt S, ThurÃ³czy J, et al. Adipose tissue macrophages in non-rodent mammals: a comparative study. Cell Tissue Res. 2016;363:461-78 pubmed publisher
Nakajima H, Kubo T, Ihara H, Hikida T, Danjo T, Nakatsuji M, et al. Nuclear-translocated Glyceraldehyde-3-phosphate Dehydrogenase Promotes Poly(ADP-ribose) Polymerase-1 Activation during Oxidative/Nitrosative Stress in Stroke. J Biol Chem. 2015;290:14493-503 pubmed publisher
Takikawa M, Nakamura S, Ishihara M, Takabayashi Y, Fujita M, Hattori H, et al. Improved angiogenesis and healing in crush syndrome by fibroblast growth factor-2-containing low-molecular-weight heparin (Fragmin)/protamine nanoparticles. J Surg Res. 2015;196:247-57 pubmed publisher
Matsui T, Nakashima S, Nishino Y, Ojima A, Nakamura N, Arima K, et al. Dipeptidyl peptidase-4 deficiency protects against experimental diabetic nephropathy partly by blocking the advanced glycation end products-receptor axis. Lab Invest. 2015;95:525-33 pubmed publisher
Chao T, Chang M, Su S, Su S. Inducible nitric oxide synthase mediates MG132 lethality in leukemic cells through mitochondrial depolarization. Free Radic Biol Med. 2014;74:175-87 pubmed publisher
Nakayama T, Fujita M, Ishihara M, Ishihara M, Ogata S, Yamamoto Y, et al. Improved survival rate by temperature control at compression sites in rat model of crush syndrome. J Surg Res. 2014;188:250-9 pubmed publisher
Grutzmacher C, Park S, Zhao Y, Morrison M, Sheibani N, Sorenson C. Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes. Am J Physiol Renal Physiol. 2013;304:F19-30 pubmed publisher
Kim H, Patel K, Muldoon Jacobs K, Bisht K, Aykin Burns N, Pennington J, et al. SIRT3 is a mitochondria-localized tumor suppressor required for maintenance of mitochondrial integrity and metabolism during stress. Cancer Cell. 2010;17:41-52 pubmed publisher

product information

Catalog No :

SMC-154D

Product Name :

Nitrotyrosine Antibody

Description :

Mouse Anti- Nitrotyrosine Monoclonal IgG2a

Target :

Nitrotyrosine

Conjugate :

Unconjugated

2021 List Price :

312.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Cell Signaling Post-translational Modifications Nitration Neuroscience Neurodegeneration Alzheimer's Disease Parkinson's Disease

Alternative Name(s) :

Nitro tyrosine Antibody, 3-Nitrotyrosine Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Trademark :

MOLECULAR SIGNATURE®

Clone Number :

39B6

Immunogen :

3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Applications :

WB IHC ICC/IF IP ELISA FCM AM

Host Species :

Mouse

Isotype :

IgG2a

Species Reactivity Abbreviation :

ALL

Species Reactivity Full Name :

Species Independent

Antibody Dilution :

WB (1:1400), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-154D, RRID: AB_904533

Certificate of Analysis :

0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Scientific Background :

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

References :

1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-154_Nitrotyrosine_Antibody_39B6_IHC_Mouse_inflamed-colon_40x_1.png SMC-154_Nitrotyrosine_Antibody_39B6_IHC_Rat_liver-tissue_1.png SMC-154_Nitrotyrosine_Antibody_39B6_IHC_Mouse_backskin_1.png SMC-154_Nitrotyrosine_Antibody_39B6_IHC_Human_colon-carcinoma_40x_1.png SMC-154_Nitrotyrosine_Antibody_39B6_WB_Human_A549-cells_1.png
SMC-154_Nitrotyrosine_Antibody_39B6_IHC
_Mouse_Retinal-Injury-Model_1.png

more info or order :

StressMarq Biosciences product webpage