p38 alpha (MAP Kinase) Antibody | StressMarq Biosciences SMC-152D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p38 alpha (MAP Kinase) Antibody

catalog :

SMC-152D

quantity :

100 µg

price :

242.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9F12

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-152D

Product Name :

p38 alpha (MAP Kinase) Antibody

Description :

Mouse Anti-Human p38 alpha (MAP Kinase) Monoclonal IgG1

Target :

p38 MAPK

Conjugate :

Unconjugated

2021 List Price :

242.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation Cancer

Alternative Name(s) :

CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

9F12

Immunogen :

Full length recombinant protein expressed in E.coli cells

Immunogen Species :

Human

Accession Number :

NP_001306.1

Swiss-Prot :

Q16539

Applications :

WB IHC IP ELISA AM

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~38kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-152D, RRID: AB_2141876

Certificate of Analysis :

Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

References :

1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-152_p38-MAPK_Antibody_9F12_IHC_Mouse_Retinal-Injury-Model_1.png SMC-152_p38-MAPK_Antibody_9F12_IHC_Human_colon-carcinoma_40x_1.png SMC-152_p38-MAPK_Antibody_9F12_WB_Human_Cell-lysates_1.png

more info or order :

StressMarq Biosciences product webpage