HSC70 (HSP73) Antibody | StressMarq Biosciences SMC-151B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSC70 (HSP73) Antibody

catalog :

SMC-151B

quantity :

200 µg

price :

330.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F2-H5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, proximity ligation assay

more info or order :

StressMarq Biosciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; rat; 1:100 western blot; rat; 1:1000	Shimomura H, Imai A, Nashida T. Characterization of cysteine string protein in rat parotid acinar cells. Arch Biochem Biophys. 2013;538:1-5 pubmed publisher
ELISA; human	Baaklini I, Wong M, Hantouche C, Patel Y, Shrier A, Young J. The DNAJA2 substrate release mechanism is essential for chaperone-mediated folding. J Biol Chem. 2012;287:41939-54 pubmed publisher
	Katoh H, Kubota T, Nakatsu Y, Tahara M, Kidokoro M, Takeda M. Heat Shock Protein 90 Ensures Efficient Mumps Virus Replication by Assisting with Viral Polymerase Complex Formation. J Virol. 2017;91: pubmed publisher
	Katoh H, Kubota T, Kita S, Nakatsu Y, Aoki N, Mori Y, et al. Heat shock protein 70 regulates degradation of the mumps virus phosphoprotein via the ubiquitin-proteasome pathway. J Virol. 2015;89:3188-99 pubmed publisher
	Kawano F, Oke Y, Nomura S, Fujita R, Ohira T, Nakai N, et al. Responses of HSC70 expression in diencephalon to iron deficiency anemia in rats. J Physiol Sci. 2011;61:445-56 pubmed publisher

product information

Catalog No :

SMC-151B

Product Name :

HSC70 (HSP73) Antibody

Description :

Mouse Anti-Human HSC70 (HSP73) Monoclonal IgG2a Kappa

Target :

HSC70 (HSP73)

Conjugate :

Unconjugated

2021 List Price :

330.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins

Alternative Name(s) :

HSC54 Antibody, HSC71 Antibody, HSC73 Antibody, HSP71 Antibody, HSP73 Antibody, HSPA10 Antibody, HSPA8 Antibody, LAP1 Antibody, NIP71 Antibody

Size :

200 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

1F2-H5

Immunogen :

Full length human HSC70

Immunogen Species :

Human

Accession Number :

NP_006588.1

Swiss-Prot :

P11142

Applications :

WB IHC ICC/IF IP ELISA PLA PBA AM

Host Species :

Mouse

Isotype :

IgG2a Kappa

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~73kDa. Does not cross react with HSP70.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-151B, RRID: AB_2120165

Certificate of Analysis :

1 µg/ml of SMC-151 was sufficient for detection of HSC70 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Melanosome

Scientific Background :

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).

References :

1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993)J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989), Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Nut1Acad. Sci. USA 89: 7290-7294. 6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-151_Hsc70-Hsp73_Antibody_1F2-H5_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SMC-151_Hsc70_Antibody_1F2-H5_WB_Human_Cell-lysates_1.png SMC-151_Hsc70-Hsp73_Antibody_1F2-H5_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SMC-151_Hsc70_Antibody_1F2-H5_ICC-IF_Human_HaCaT-cells_1.png

more info or order :

StressMarq Biosciences product webpage