product summary
Loading...
company name :
StressMarq Biosciences
product type :
antibody
product name :
HSP40, YDJ1 Antibody
catalog :
SMC-150D
quantity :
100 µg
price :
294.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1G10.H8
reactivity :
Saccharomyces cerevisiae S288C
application :
western blot, ELISA, immunoprecipitation
more info or order :
image
image 1 :
Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8 (SMC-150). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-150) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
product information
Catalog No :
SMC-150D
Product Name :
HSP40, YDJ1 Antibody
Description :
Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa
Target :
HSP40
Conjugate :
Unconjugated
2021 List Price :
294.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins
Alternative Name(s) :
DNAJA2 Antibody, CPR3 Antibody, HIRIP4 Antibody, DNAJ Antibody, DNJ3 Antibody, DJ3 Antibody, RDJ2 Antibody, HIRa interacting protein4 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
1G10.H8
Immunogen :
Full length protein yeast HSP40 (YDJ1)
Immunogen Species :
Yeast
Accession Number :
NP_014335.1
Swiss-Prot :
P25491
Applications :
WB IP ELISA
Host Species :
Mouse
Isotype :
IgG1 Kappa
Species Reactivity Abbreviation :
Ys
Species Reactivity Full Name :
Yeast
Antibody Dilution :
WB (1:2000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~40kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-150D, RRID: AB_1250176
Certificate of Analysis :
0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus
Scientific Background :
Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not.
Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
References :
1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.
3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.
4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.
7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-150_Hsp40_Antibody_1G10-H8_WB_Human_Cell-lysates_1.png
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
related products
browse more products
questions and comments