antibody

LAMP2 Antibody

SMC-141D

100 µg

279.00 USD

monoclonal

rat

nonconjugated

GL2A7

human, mouse

western blot, immunocytochemistry, immunoprecipitation

LAMP2 Antibody

Rat Anti-Mouse LAMP2 Monoclonal IgG1

LAMP2

Unconjugated

279.00 USD

USD

Cell Signaling Tags and Cell Markers Organelle Markers Protein Trafficking Chaperone Proteins Neuroscience

CD107b Antibody, Igp110 Antibody, Igp2 Antibody, Lamp2C Antibody, LampB Antibody, MAC3 Antibody, Lysosome-associated membrane glycoprotein 2 Antibody, LAMP-2 Antibody, Lysosome-associated membrane protein 2 Antibody, CD107 antigen-like family member B Antibody, Lysosomal membrane glycoprotein type B Antibody, LGP-B Antibody

100 µg

Antibodies

Monoclonal

GL2A7

Purified preparation of mouse liver lysosomal membranes

Mouse

NP_001017959.1

P17047

WB ICC/IF IP

Rat

IgG1

Hu Ms Rb

Human Mouse Rabbit

WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.

Protein G Purified

PBS pH7.4, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects ~100-110kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-141D, RRID: AB_2281134

1 µg/ml of SMC-141 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cell Membrane Endosome Lysosome Endosome membrane Lysosome membrane

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043. 2. Furuta K., et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer J., et al. (1996) J Cell Biol. 132(4): 565-76. 4. Lichter-Konecki U., et al (1999) Differentiation 65(1): 43-58. 5. Grutzkau A., et al. (2004) Cytometry A. 61(1): 62-68. 6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19. 7. Tanaka Y., et al. (2000) Nature 406: 902-906.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SMC-141_LAMP2_Antibody_GL2A7_ICC-IF_Rabbit_Corneal-Endothelial-Cell-CEC_1.png SMC-141_LAMP2_Antibody_GL2A7_WB_Human-Mouse_HEK293-and-3T3NIH-cell-lysates_1.png