LAMP1 Antibody | StressMarq Biosciences SMC-140D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

LAMP1 Antibody

catalog :

SMC-140D

quantity :

100 µg

price :

291.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ly1C6

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse	Harada Y, Hiasa M. Immunological identification of vesicular nucleotide transporter in intestinal L cells. Biol Pharm Bull. 2014;37:1090-5 pubmed
immunocytochemistry; human	Hiasa M, Togawa N, Miyaji T, Omote H, Yamamoto A, Moriyama Y. Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets. Physiol Rep. 2014;2: pubmed publisher
	Harada Y, Kato Y, Miyaji T, Omote H, Moriyama Y, Hiasa M. Vesicular nucleotide transporter mediates ATP release and migration in neutrophils. J Biol Chem. 2018;293:3770-3779 pubmed publisher

product information

Catalog No :

SMC-140D

Product Name :

LAMP1 Antibody

Description :

Mouse Anti-Rat LAMP1 Monoclonal IgG1

Target :

LAMP1

Conjugate :

Unconjugated

2021 List Price :

291.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Tags and Cell Markers Organelle Markers Protein Trafficking Chaperone Proteins Neuroscience

Alternative Name(s) :

CD107 Antibody, CD107a Antibody, LAMPA Antibody, LGP120 Antibody, IgpA Antibody, Lysosome-associated membrane glycoprotein 1 Antibody, CD107 antigen-like family member A Antibody, 120 kDa lysosomal membrane glycoprotein Antibody, Lamp-1 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

Ly1C6

Immunogen :

Rat liver lysosomal membrane preparations

Immunogen Species :

Rat

Accession Number :

NP_036989.1

Swiss-Prot :

P14562

Applications :

WB ICC/IF IP

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt Hm

Species Reactivity Full Name :

Human Mouse Rat Hamster

Antibody Dilution :

WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~120kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-140D, RRID: AB_2234507

Certificate of Analysis :

1 µg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.

Cellular Localization :

Cell Membrane Endosome Lysosome Endosome membrane Lysosome membrane

Scientific Background :

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

References :

1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68. 2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76. 4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19. 5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847. 6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-140_LAMP1_Antibody_Ly1C6_ICC-IF_Human_transfected-HeLa-cells_1.png SMC-140_LAMP1_Antibody_Ly1C6_WB_Rat_liver-micosome-lysate_1.png

more info or order :

StressMarq Biosciences product webpage