FKBP52 Antibody | StressMarq Biosciences SMC-139D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

FKBP52 Antibody

catalog :

SMC-139D

quantity :

100 µg

price :

314.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Hi52C

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 1

Reference
Bachman A, Keramisanou D, Xu W, Beebe K, Moses M, Vasantha Kumar M, et al. Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation. Nat Commun. 2018;9:265 pubmed publisher

product information

Catalog No :

SMC-139D

Product Name :

FKBP52 Antibody

Description :

Mouse Anti-Human FKBP52 Monoclonal IgG2a

Target :

FKBP52

Conjugate :

Unconjugated

2021 List Price :

314.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Nuclear Import/Export

Alternative Name(s) :

FK506 binding protein 4 Antibody, FKBP4 Antibody, FKBP59 Antibody, HBI Antibody, HSP56 Antibody, p52 Antibody, p59 Antibody, PPIase Antibody, Rotamase Antibody, T cell FK506 binding protein Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

Hi52C

Immunogen :

Synthetic peptide corresponding to the residues of human FKBP52

Immunogen Species :

Human

Accession Number :

NP_002005.1

Swiss-Prot :

Q02790

Applications :

WB IHC ICC/IF IP

Host Species :

Mouse

Isotype :

IgG2a

Species Reactivity Abbreviation :

Hu Ms Rt Dg Hm

Species Reactivity Full Name :

Human Mouse Rat Dog Hamster

Antibody Dilution :

WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-139D, RRID: AB_2293934

Certificate of Analysis :

0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

References :

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7):3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-139_FKBP52_Antibody_Hi52C_IHC_Mouse_backskin_1.png SMC-139_FKBP52_Antibody_Hi52C_WB_Rat_Brain-Heart-Kidney-Liver-Pancreas-Skeletal-muscle-Spleen-Testes-Thymus-cell-lysates_1.png SMC-139_FKBP52_Antibody_Hi52C_ICC-IF_Human_MCF-7-cells-metastatic-mammary-gland-breast-cell-line_1.png SMC-139_FKBP52_Antibody_Hi52C_WB_Human_Cell-lysates_1.png
SMC-139_FKBP52_Antibody_Hi52C_IHC_Human
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ls_1.png

more info or order :

StressMarq Biosciences product webpage