HSP90 beta Antibody | StressMarq Biosciences SMC-136D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP90 beta Antibody

catalog :

SMC-136D

quantity :

100 µg

price :

314.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Hyb-K3701

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human	Muralidharan S, Ambade A, Fulham M, Deshpande J, Catalano D, Mandrekar P. Moderate alcohol induces stress proteins HSF1 and hsp70 and inhibits proinflammatory cytokines resulting in endotoxin tolerance. J Immunol. 2014;193:1975-87 pubmed publisher
western blot; human	Bates J, Salzman J, May D, García P, Hogan G, McIntosh M, et al. Extensive gene-specific translational reprogramming in a model of B cell differentiation and Abl-dependent transformation. PLoS ONE. 2012;7:e37108 pubmed publisher
	Rozenberg P, Ziporen L, Gancz D, Saar Ray M, Fishelson Z. Cooperation between Hsp90 and mortalin/GRP75 in resistance to cell death induced by complement C5b-9. Cell Death Dis. 2018;9:150 pubmed publisher

product information

Catalog No :

SMC-136D

Product Name :

HSP90 beta Antibody

Description :

Mouse Anti-Human HSP90 beta Monoclonal IgM

Target :

HSP90 beta

Conjugate :

Unconjugated

2021 List Price :

314.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

Alternative Name(s) :

HSP84 Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody, D6S182. FLJ26984 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

Hyb-K3701

Immunogen :

Recombinant human HSP90beta; Specificity mapped to amino acids 185-335

Immunogen Species :

Human

Accession Number :

NP_031381.2

Swiss-Prot :

P08238

Applications :

WB IHC ELISA

Host Species :

Mouse

Isotype :

IgM

Species Reactivity Abbreviation :

Hu Ms

Species Reactivity Full Name :

Human Mouse

Antibody Dilution :

WB (1:1000), IHC (1:3000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects 90kDa. This is a beta specific product, does not cross-react with alpha isoforms.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-136D, RRID: AB_2121403

Certificate of Analysis :

1 µg/ml was sufficient for detection of HSP90β in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Cellular Localization :

Cytoplasm Melanosome

Scientific Background :

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References :

1. Nemoto, T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4): 296-311.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-136_Hsp90-beta_Antibody_K3701_IHC_Mouse_inflamed-colon_40x_1.png SMC-136_Hsp90-beta_Antibody_K3701_IHC_Human_colon-carcinoma_40x_1.png SMC-136_Hsp90-beta_Antibody_K3701_WB_Human_Cell-lysates_1.png

more info or order :

StressMarq Biosciences product webpage