HSP90 alpha/beta Antibody | StressMarq Biosciences SMC-135D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP90 alpha/beta Antibody

catalog :

SMC-135D

quantity :

100 µg

price :

314.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Hyb-K41220A

reactivity :

human, mouse, rat, brewer's yeast

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast	Horvat N, Armstrong H, Lee B, Mercier R, Wolmarans A, Knowles J, et al. A mutation in the catalytic loop of Hsp90 specifically impairs ATPase stimulation by Aha1p, but not Hch1p. J Mol Biol. 2014;426:2379-92 pubmed publisher
	PeriÄ‡ M, Bou Dib P, Dennerlein S, Musa M, Rudan M, LovriÄ‡ A, et al. Crosstalk between cellular compartments protects against proteotoxicity and extends lifespan. Sci Rep. 2016;6:28751 pubmed publisher
	Stasyk T, Holzmann J, Stumberger S, Ebner H, Hess M, Bonn G, et al. Proteomic analysis of endosomes from genetically modified p14/MP1 mouse embryonic fibroblasts. Proteomics. 2010;10:4117-27 pubmed publisher

product information

Catalog No :

SMC-135D

Product Name :

HSP90 alpha/beta Antibody

Description :

Mouse Anti-Human HSP90 alpha/beta Monoclonal IgG2a

Target :

HSP90 alpha/beta

Conjugate :

Unconjugated

2021 List Price :

314.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

Alternative Name(s) :

HSP86 Antibody, HSP89A Antibody, HSP90AA1 Antibody, HSP90Alpha Antibody, HSPC1 Antibody, HSPCA Antibody, HSPCAL3 Antibody, HSP84 Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody, D6S182 Antibody, FLJ26985 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

Hyb-K41220A

Immunogen :

Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304

Immunogen Species :

Human

Accession Number :

NP_031381.2, NP_001017963.2

Swiss-Prot :

P08238, P07900

Applications :

WB IHC ICC/IF ELISA

Host Species :

Mouse

Isotype :

IgG2a

Species Reactivity Abbreviation :

Hu Ms Rt Ys

Species Reactivity Full Name :

Human Mouse Rat Yeast Yeast (Saccharomyces cerevisiae) Fisson Yeast (Schizosaccharomyces pombe)

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-135D, RRID: AB_2121063

Certificate of Analysis :

1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Cellular Localization :

Cytoplasm Melanosome

Scientific Background :

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References :

1. Nemoto, T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4): 296-311.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-135_Hsp90-alpha_Antibody_K41220A_WB_Human_Cell-lysates_1.png SMC-135_Hsp90-alpha-beta_Antibody_K41220A_ICC-IF_Human_HeLa-Cells_20x_Composite.png
SMC-135_Hsp90-alpha_Antibody_K41220A_IH
C_Mouse_backskin_1.png
SMC-135_Hsp90-alpha-beta_Antibody_K41220A_ICC-IF_Human_HeLa-Cells_100x_Composite.png

more info or order :

StressMarq Biosciences product webpage