Grp75 Antibody | StressMarq Biosciences SMC-133D product information

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product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Grp75 Antibody

catalog :

SMC-133D

quantity :

100 µg

price :

296.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S52A-42

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human western blot; human	Saar Ray M, Moskovich O, Iosefson O, Fishelson Z. Mortalin/GRP75 binds to complement C9 and plays a role in resistance to complement-dependent cytotoxicity. J Biol Chem. 2014;289:15014-22 pubmed publisher
ELISA; human	Rozenberg P, Kocsis J, Saar M, Prohaszka Z, Fust G, Fishelson Z. Elevated levels of mitochondrial mortalin and cytosolic HSP70 in blood as risk factors in patients with colorectal cancer. Int J Cancer. 2013;133:514-8 pubmed publisher
	Rozenberg P, Ziporen L, Gancz D, Saar Ray M, Fishelson Z. Cooperation between Hsp90 and mortalin/GRP75 in resistance to cell death induced by complement C5b-9. Cell Death Dis. 2018;9:150 pubmed publisher
	Hillman Y, Mazkereth N, Farberov L, Shomron N, Fishelson Z. Regulation of Complement-Dependent Cytotoxicity by MicroRNAs miR-200b, miR-200c, and miR-217. J Immunol. 2016;196:5156-65 pubmed publisher

product information

Catalog No :

SMC-133D

Product Name :

Grp75 Antibody

Description :

Mouse Anti-Mouse Grp75 Monoclonal IgG1

Target :

GRP75

Conjugate :

Unconjugated

2021 List Price :

296.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Neuroscience Neurodegeneration Alzheimer's Disease Parkinson's Disease Neuroscience Neurodegeneration Alzheimer's Disease Cell Signaling Metabolism Mitochondrial Metabolism Mitochondrial Markers Cancer Cell Cycle Cell Division Cancer Tumor Biomarkers

Alternative Name(s) :

HSC74 Antibody, HSP74 Antibody, HSPA9 Antibody, HSPa9a Antibody, HSPA9B Antibody, Mortalin 2 Antibody, MOT2 Antibody, PBP74 Antibody, 75 kDa glucose regulated protein antibody, 75 kDa glucose-regulated protein antibody, CSA antibody, Glucose Regulated Protein antibody, Grp 75 antibody, GRP-75 antibody, GRP75 antibody, GRP75_HUMAN antibody, Heat shock 70 kDa protein 9 antibody, Heat shock 70kD protein 9 antibody, heat shock 70kDa protein 9 antibody, Heat shock 70kDa protein 9B antibody, Heat shock protein 74 kDa A antibody, Heat shock protein A antibody, Heat shock protein cognate 74 antibody, Hsc74 antibody, Hsp74 antibody, Hsp74a antibody, HSPA9 antibody, Hspa9a antibody, HSPA9B antibody, MGC4500 antibody, mitochondrial antibody, Mortalin 2 antibody, Mortalin antibody, Mortalin perinuclear antibody, Mortalin2 antibody, MOT 2 antibody, MOT antibody, MOT2 antibody, Mthsp70 antibody, p66 mortalin antibody, P66 MOT antibody, PBP74 antibody, Peptide binding protein 74 antibody, Peptide-binding protein 74 antibody, Stress 70 protein mitochondrial antibody, Stress 70 protein mitochondrial precursor antibody, Stress-70 protein antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

S52A-42

Immunogen :

Fusion protein amino acids 551-766 of mouse SALM2.

Immunogen Species :

Mouse

Accession Number :

NP_004125.3

Swiss-Prot :

P38646

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt C.el

Species Reactivity Full Name :

Human Mouse Rat Nematode (Caenorhabditis elegans)

Antibody Dilution :

WB (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~75kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-133D, RRID: AB_2120318

Certificate of Analysis :

1 µg/ml was sufficient for detection of Grp75 in 10 µg of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Cellular Localization :

Mitochondrion

Scientific Background :

Grp75, also known as mortalin, is a member of HSP70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with HSP60 in the re-folding of proteins translocated into this organelle (3, 4). Related forms may also be found in the cytosol or on the surface of the extracellular membrane. Other Grp75 functions include its ability to inactivate the tumor suppressor p53 (5). Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (6). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (7). And finally, like its E.coli homolog DnaK (8), GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone (9, 10).

References :

1. Kaul S.C., et al. (1993) Biochem Biophys Res Commun. 193: 348-355. 2. Wadhwa R., et al. (1993) J Biol Chem 268: 6615-6621. 3. Schneider H.C., et al. (1994) Nature 371: 768-774. 4. Manning-Krieg U.C., et al. (1991) EMBO J. 10: 3273-3280. 5. Wadhwa R., et al. (1998) J Biol Chem. 273: 29586-91. 6. Wadhwa R., et al. (2006) Int J Cancer 118: 2973-2980. 7. Kaul S.C., et al. (2003) Exp Cell Res. 286: 96-110. 8. Liberek K., et al. (1991) J Biol Chem. 266: 14491-14496. 9. Mizzen L.A., et al. (1991) Cell Regulation. 2: 165-179. 10. Leustek U.K., et al. (1989) PNAS USA. 86: 7805-7808.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-133_Grp75_Antibody_S52A-42_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SMC-133_Grp75_Antibody_S52A-42_WB_Human_HeLa-cell-lysates_1.png SMC-133_Grp75_Antibody_S52A-42_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png