HO-1 Antibody | StressMarq Biosciences SMC-131D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HO-1 Antibody

catalog :

SMC-131D

quantity :

100 µg

price :

304.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F12-A6

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse	Al Rifai N, Rücker H, Amslinger S. Opening or closing the lock? When reactivity is the key to biological activity. Chemistry. 2013;19:15384-95 pubmed publisher
	Wang H, Zhang Y, Xia F, Zhang W, Chen P, Yang G. Protective effect of silencing Stat1 on high glucose-induced podocytes injury via Forkhead transcription factor O1-regulated the oxidative stress response. BMC Mol Cell Biol. 2019;20:27 pubmed publisher
	Yamaguchi Y, Kanzaki H, Katsumata Y, Itohiya K, Fukaya S, Miyamoto Y, et al. Dimethyl fumarate inhibits osteoclasts via attenuation of reactive oxygen species signalling by augmented antioxidation. J Cell Mol Med. 2018;22:1138-1147 pubmed publisher
	Kanzaki H, Shinohara F, Itohiya Kasuya K, Ishikawa M, Nakamura Y. Nrf2 activation attenuates both orthodontic tooth movement and relapse. J Dent Res. 2015;94:787-94 pubmed publisher
	Kanzaki H, Shinohara F, Kajiya M, Fukaya S, Miyamoto Y, Nakamura Y. Nuclear Nrf2 induction by protein transduction attenuates osteoclastogenesis. Free Radic Biol Med. 2014;77:239-48 pubmed publisher

product information

Catalog No :

SMC-131D

Product Name :

HO-1 Antibody

Description :

Mouse Anti-Human HO-1 Monoclonal IgG1 Kappa

Target :

HO-1

Conjugate :

Unconjugated

2021 List Price :

304.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Neuroscience Neurodegeneration Alzheimer's Disease Cardiovascular System Blood Platelets Cell Signaling Epigenetics and Nuclear Signaling Nuclear Signaling Pathways NFkB Pathway Cardiovascular System Atherosclerosis Vascular Inflammation Inflammatory Mediators Cardiovascular System Vasculature Endothelium Cancer Cancer Metabolism Response to Hypoxia Cell Signaling Metabolism Metabolism processes Hypoxia

Alternative Name(s) :

HSP32 Antibody, HMOX1 Antibody, Heme oxygenase 1 Antibody, HO Antibody, HO1 Antibody, , 32 kD antibody, bK286B10 antibody, D8Wsu38e antibody, heat shock protein 32 kD antibody, heat shock protein 32kD antibody, Heat shock protein antibody, Heme oxygenase (decycling) 1 antibody, Heme oxygenase 1 antibody, Hemox antibody, HMOX 1 antibody, Hmox antibody, Hmox1 antibody, HMOX1_HUMAN antibody, HO 1 antibody, HO antibody, HO-1 antibody, HO1 antibody, Hsp32 antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

1F12-A6

Immunogen :

Human HO-1 synthetic peptide, amino acids 1-30

Immunogen Species :

Human

Accession Number :

NP_002124.1

Swiss-Prot :

P09601

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms Rt Bv Dg GP Hm Mk Pg Rb

Species Reactivity Full Name :

Human Mouse Rat Bovine Dog Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects 32kDa. Does not cross-react with HO-2.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-131D, RRID: AB_2264116

Certificate of Analysis :

1 µg/ml was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Cellular Localization :

Microsome Endoplasmic Reticulum

Scientific Background :

Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

References :

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-131_HO-1_Antibody_1F12-A6_ICC-IF_Human_HeLa-Cells_100x_Composite.png SMC-131_HO-1_Antibody_1F12-A6_IHC_Mouse_backskin_6.png SMC-131_HO-1_Antibody_1F12-A6_ICC-IF_Human_HeLa-Cells_20x_Composite.png SMC-131_HO-1_Antibody_1F12-A6_WB_Human_HeLa-cell-lysates_1.png SMC-131_HO-1_Antibody_1F12-A6_ICC-IF_Human_HaCaT-cells_2.png

more info or order :

StressMarq Biosciences product webpage