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company name :
StressMarq Biosciences
product type :
antibody
product name :
KDEL Receptor Antibody
catalog :
SMC-129D
quantity :
100 µg
price :
286.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
KR-10
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-129D
Product Name :
KDEL Receptor Antibody
Description :
Mouse Anti-Bovine KDEL Receptor Monoclonal IgG1
Target :
KDEL Receptor
Conjugate :
Unconjugated
2021 List Price :
286.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Tags and Cell Markers Organelle Markers Protein Trafficking Chaperone Proteins
Alternative Name(s) :
ERD2 Antibody, ERD2.1 Antibody, ERD21 Antibody, HDEL Antibody, KDEL Antibody, KDEL R1 Antibody, KDELR1 Antibody, PM23 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
KR-10
Immunogen :
A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
Immunogen Species :
Bovine
Accession Number :
NP_001069963.1
Swiss-Prot :
P33946
Applications :
WB IHC ICC/IF IP
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt Bv Mk Hm Rb Pg Sh Dg Ck Dr Xe
Species Reactivity Full Name :
Human Mouse Rat Bovine Monkey Hamster Rabbit Pig Sheep Dog Chicken Fruit Fly (Drosophila melanogaster) African clawed frog (Xenopus laevis)
Antibody Dilution :
WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~25kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-129D, RRID: AB_2132188
Certificate of Analysis :
1 µg/ml was sufficient for detection of KDEL receptor in 20 µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Cellular Localization :
Endoplasmic Reticulum
Scientific Background :
The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
References :
1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737. 2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219. 3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316. 4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328. 5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163. 6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-129_KDEL-Receptor_Antibody_KR-10_ICC-IF_Rat_NRK-cells_1.png SMC-129_KDEL-Receptor_Antibody_KR-10_WB_Rat_tissue-lysate_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.