CaMKII Antibody | StressMarq Biosciences SMC-125D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

CaMKII Antibody

catalog :

SMC-125D

quantity :

100 µg

price :

278.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

22B1

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500	Feng T, Szabo E, Dziak E, Opas M. Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells. Stem Cell Rev. 2010;6:74-85 pubmed publisher

product information

Catalog No :

SMC-125D

Product Name :

CaMKII Antibody

Description :

Mouse Anti-Rat CaMKII Monoclonal IgG1

Target :

CaMKII

Conjugate :

Unconjugated

2021 List Price :

278.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation

Alternative Name(s) :

CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

22B1

Immunogen :

Synthetic peptide

Immunogen Species :

Rat

Accession Number :

NP_037052.1

Swiss-Prot :

P11275

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-125D, RRID: AB_2067924

Certificate of Analysis :

1 µg/ml was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Cellular Localization :

Cytoplasm Mitochondrion Nucleus Cell Junction Synapse Presynaptic Cell Membrane

Scientific Background :

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

References :

1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-125_CaMKII_Antibody_22B1_IHC_Human_colon-carcinoma_40x_1.png SMC-125_CaMKII_Antibody_22B1_WB_Mouse_Ventricle-lysates_1.png
SMC-125_CaMKII_Antibody_22B1_IHC_Mouse_
backskin_8.png
SMC-125_CaMKII_Antibody_22B1_ICC-IF_Rat_dissociated-hippocampal-neurons_10X_1.png

more info or order :

StressMarq Biosciences product webpage