PSD95 Antibody | StressMarq Biosciences SMC-122D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

PSD95 Antibody

catalog :

SMC-122D

quantity :

100 µg

price :

278.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6G6

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-122D

Product Name :

PSD95 Antibody

Description :

Mouse Anti-Rat PSD95 Monoclonal IgG2a

Target :

PSD95

Conjugate :

Unconjugated

2021 List Price :

278.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Cell Markers Neuron Markers Postsynaptic Markers Cell Signaling Organelle Markers

Alternative Name(s) :

PSD 95 Antibody, PSD-95 Antibody, DLG4 Antibody, SAP90 Antibody, Synapse-associated protein 90 Antibody, Postsynaptic density protein 95 Antibody, Disks large homolog 4 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

6G6

Immunogen :

Recombinant rat PSD-95

Immunogen Species :

Rat

Accession Number :

NP_062567.1

Swiss-Prot :

P31016

Applications :

WB IHC ICC/IF AM

Host Species :

Mouse

Isotype :

IgG2a

Species Reactivity Abbreviation :

Hu Ms Rt Bv

Species Reactivity Full Name :

Human Mouse Rat Bovine

Antibody Dilution :

WB (1:250), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-122D, RRID: AB_2300386

Certificate of Analysis :

1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

Cellular Localization :

Cell Membrane Cell Junction Synapse Postsynaptic Cell Membrane Postsynaptic Density Cell Projection Axon

Scientific Background :

Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).

References :

1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25. 2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277(5331): 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-122_PSD95_Antibody_6G6_ICC-IF_Human_HaCaT-cells_1.png SMC-122_PSD95_Antibody_6G6_ICC-IF_Rat_dissociated-hippocampal-neurons_10X_1.png SMC-122_PSD95_Antibody_6G6_WB_Rat_brain-membrane-lysate_1.png

more info or order :

StressMarq Biosciences product webpage