HSF2 Antibody | StressMarq Biosciences SMC-119D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSF2 Antibody

catalog :

SMC-119D

quantity :

100 µg

price :

231.00 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3E2

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-119D

Product Name :

HSF2 Antibody

Description :

Rat Anti-Mouse HSF2 Monoclonal IgG1

Target :

HSF2

Conjugate :

Unconjugated

2021 List Price :

231.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Epigenetics and Nuclear Signaling

Alternative Name(s) :

HSTF2 Antibody, Heat shock factor protein 2 Antibody, Heat shock transcription factor 2 Antibody, HSF 2 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

3E2

Immunogen :

Purified recombinant mouse HSF2 protein

Immunogen Species :

Mouse

Accession Number :

NP_001129036.1

Swiss-Prot :

P38533

Applications :

WB ICC/IF AM

Host Species :

Rat

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt GP Hm Mk Rb Dg Bv Sh Pg

Species Reactivity Full Name :

Human Mouse Rat Guinea Pig (Cavia porcellus) Hamster Monkey Rabbit Dog Bovine Sheep Pig

Antibody Dilution :

WB (1:250), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~69kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-119D, RRID: AB_2264323

Certificate of Analysis :

4 µg/ml of SMC-119 was sufficient for detection of HSF2 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

References :

1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3.Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774. 7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334- 1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-119_HSF2_Antibody_3E2_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SMC-119_HSF2_Antibody_3E2_WB_Human_K562-cell-lysates_1.png SMC-119_HSF2_Antibody_3E2_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

more info or order :

StressMarq Biosciences product webpage