antibody

CD74 Antibody

SMC-116D

100 µg

241.00 USD

monoclonal

mouse

nonconjugated

PIN.1

human, mouse

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

CD74 Antibody

Mouse Anti-Human CD74 Monoclonal IgG1

CD74

Unconjugated

241.00 USD

USD

Immunology Adaptive Immunity B Cells B Cell CD Markers

DHLAG Antibody, HLA DR gamma Antibody, HLADG Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody, CD 74 antibody, CD74 antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 antigen antibody, CD74 molecule antibody, CD74 molecule, major histocompatibility complex, class II invariant chain antibody, CLIP antibody, DHLAG antibody, Gamma chain of class II antigens antibody, HG2A_HUMAN antibody, HLA class II histocompatibility antigen gamma chain antibody, HLA DR antigens associated invariant chain antibody, HLA DR gamma antibody, HLA-DR antigens-associated invariant chain antibody, HLA-DR-gamma antibody, HLADG antibody, HLADR antigens associated invariant chain antibody Ia antigen associated invariant chain antibody, Ia antigen-associated invariant chain antibody, Ia associated invariant chain antibody, Ia gamma antibody, Ii antibody, Invariant polypeptide of major histocompatibility complex class II antigen associated antibody, la-gamma antibody, Major histocompatibility complex class II invariant chain antibody, MHC HLA DR gamma chain antibody, MHC HLA-DR gamma chain antibody, p33 antibody, p35 antibody, Protein 41 antibody

100 µg

Antibodies

Monoclonal

PIN.1

Human CD74 invariant chain synthetic peptide

Human

NP_001020329.1

P04233

WB IHC ICC/IF IP FCM FACS

Mouse

IgG1

Hu Ms

Human Mouse

WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Protein G Purified

PBS pH7.2, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-116D, RRID: AB_2260133

1 µg/ml of SMC-116 was sufficient for detection of CD74 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Cell Membrane Endoplasmic Reticulum Endoplasmic Reticulum Membrane Golgi Apparatus Endosome Lysosome

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9. 2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9. 3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816. 4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11. 5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165. 6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SMC-116_CD74_Antibody_PIN-1-1_ICC-IF_Human_HeLa-Cells_100x_Composite.png SMC-116_CD74_Antibody_PIN-1-1_IHC_Mouse_backskin_3.png SMC-116_CD74_Antibody_PIN-1-1_WB_Human_N87-cell-lysates_1.png SMC-116_CD74_Antibody_PIN-1-1_ICC-IF_Human_HaCaT-cells_2.png