HSP25/HSP27 Antibody | StressMarq Biosciences SMC-114D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP25/HSP27 Antibody

catalog :

SMC-114D

quantity :

100 µg

price :

254.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8A7

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

StressMarq Biosciences product webpage

citations: 1

Reference
Benndorf R, Gilmont R, Hirano S, Ransom R, Jungblut P, Bommer M, et al. Small heat shock protein speciation: novel non-canonical 44 kDa HspB5-related protein species in rat and human tissues. Cell Stress Chaperones. 2018;23:813-826 pubmed publisher

product information

Catalog No :

SMC-114D

Product Name :

HSP25/HSP27 Antibody

Description :

Mouse Anti-Human HSP25/HSP27 Monoclonal IgG1 Kappa

Target :

HSP27

Conjugate :

Unconjugated

2021 List Price :

254.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Cytoskeleton Microfilaments Actin Actin Assembly Cell Signaling Cytoskeleton Microtubules Cancer Tumor Biomarkers Cardiovascular System Atherosclerosis Cardiovascular System Heart Contractility Cancer Heat Shock

Alternative Name(s) :

HSPB1 Antibody, Heat shock protein beta-1 Antibody, HSPB1 Antibody, DMPK-binding protein Antibody, MKBP Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

8A7

Immunogen :

HSP27 peptide

Immunogen Species :

Human

Accession Number :

NP_001532.1

Swiss-Prot :

P04792

Applications :

WB IHC ICC/IF IP FCM FACS

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms Rt Bv Dg GP Hm Sh

Species Reactivity Full Name :

Human Mouse Rat Bovine Dog Guinea Pig (Cavia porcellus) Hamster Sheep

Antibody Dilution :

WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-114D, RRID: AB_2120775

Certificate of Analysis :

A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Cytoplasm Nucleus Cytoskeleton Spindle

Scientific Background :

HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.

References :

1. Welch W.J. (1985) J Biol. Chem. 260: 3058-3062. 2. Kim K.K., Kim R., and Kim S. (1998) Nature 394(6693): 595-599. 3. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 4. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 5. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 6. Welsh M.J., Wu W., Parvinem M., and Gilmont R.R. (1996) Biol. Of Reprod. 55: 141-151. 7. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 8. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6. 9. Jia, Y. et al. (2001) J. Biol. Chem. 276(43):39911-39918.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-114_Hsp27_Antibody_8A7_WB_Rat_Lung-tissue-lysates_1.png SMC-114_Hsp27_Antibody_8A7_IHC_Mouse_backskin_3.png
SMC-114_Hsp27_Antibody_8A7_IHC_Mouse_ba
ckskin_1.png
SMC-114_Hsp27_Antibody_8A7_IP_Rat_embryo-somites_1.png SMC-114_Hsp27_Antibody_8A7_IHC_Human_colon-carcinoma_40x_1.png

more info or order :

StressMarq Biosciences product webpage