antibody

HSP90 Antibody

SMC-112B

200 µg

314.00 USD

monoclonal

mouse

nonconjugated

AC-16

human, mouse, rat, chicken

western blot, immunohistochemistry

HSP90 Antibody

Mouse Anti-Water Mold HSP90 Monoclonal IgG2b

HSP90

Unconjugated

314.00 USD

USD

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

HSP84 Antibody, HSP86 Antibody, HSP90 Antibody, HSP89 Antibody, HSP90Beta Antibody, HSP90A Antibody, HSP90AA1 Antibody, HSP90AB1 Antibody, HSP90B Antibody, HSP90N Antibody, HSPC1 Antibody, HSPC2 Antibody, HSPCA Antibody, HSPCAL1 Antibody, HSPCAL4 Antibody, HSPCB Antibody, HSPN Antibody, LAP2 Antibody

200 µg

Antibodies

Monoclonal

AC-16

Heat shock protein 90 from the water mold Achyla ambisexualis

Water Mold

AAM90675.1

Q8LLI5

WB IHC AM

Mouse

IgG2b

Hu Ms Rt Rb Ck Pl Fu Is

Human Mouse Rat Rabbit Chicken Plant Fungi Water Mold (Achlya) Wheat (Triticum spp.) Insect Fall Armyworm (Spodoptera frugiperda)

WB (1:1000), IHC (1:2000); optimal dilutions for assays should be determined by the user.

Protein G Purified

PBS pH7.4, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects 90kDa. This antibody is reactive with both the constitutive and the inducible form of HSP90. It does not bind to the native form and does not recognize HSP90 from E.coli or yeast.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-112B, RRID: AB_904523

1 µg/ml of SMC-112 was sufficient for detection of HSP90 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Cytoplasm Melanosome

HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immuno-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

1. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H, et al. (2001) Adv Protein Chem 59:157-186. 3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61. 4. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133. 5. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 6. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 7. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

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