HSP60 Antibody | StressMarq Biosciences SMC-110B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP60 Antibody

catalog :

SMC-110B

quantity :

200 µg

price :

236.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LK1

reactivity :

human, mouse, rat, dogs, bovine, soybeans

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

StressMarq Biosciences product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6g	Richter F, Dennerlein S, Nikolov M, Jans D, Naumenko N, Aich A, et al. ROMO1 is a constituent of the human presequence translocase required for YME1L protease import. J Cell Biol. 2019;218:598-614 pubmed publisher
western blot; mouse	Doğan S, Pujol C, Maiti P, Kukat A, Wang S, Hermans S, et al. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell Metab. 2014;19:458-69 pubmed publisher
western blot; soybeans	Ahsan N, Donnart T, Nouri M, Komatsu S. Tissue-specific defense and thermo-adaptive mechanisms of soybean seedlings under heat stress revealed by proteomic approach. J Proteome Res. 2010;9:4189-204 pubmed publisher
western blot; soybeans	Ahsan N, Komatsu S. Comparative analyses of the proteomes of leaves and flowers at various stages of development reveal organ-specific functional differentiation of proteins in soybean. Proteomics. 2009;9:4889-907 pubmed publisher
	Nishida K, Sakakibara K, Iwasaki Y, Yamada H, Murakami R, Murota Y, et al. Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis. Nature. 2018;555:260-264 pubmed publisher
	Nonaka K, Une S, Komatsu M, Yamaji R, Akiyama J. Heat stress prevents the decrease in succinate dehydrogenase activity in the extensor digitorum longus of streptozotocin-induced diabetic rats. Physiol Res. 2018;67:117-126 pubmed
	Saita S, Nolte H, Fiedler K, Kashkar H, Venne A, Zahedi R, et al. PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis. Nat Cell Biol. 2017;19:318-328 pubmed publisher
	Seiferling D, Szczepanowska K, Becker C, Senft K, Hermans S, Maiti P, et al. Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep. 2016;17:953-64 pubmed publisher

product information

Catalog No :

SMC-110B

Product Name :

HSP60 Antibody

Description :

Mouse Anti-Human HSP60 Monoclonal IgG1

Target :

HSP60

Conjugate :

Unconjugated

2021 List Price :

236.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Tags and Cell Markers Organelle Markers

Alternative Name(s) :

CPN60 Antibody, GROEL Antibody, HLD4 Antibody, HSP 60 Antibody, HSP65 Antibody, HSPD1 Antibody, HuCHA60 Antibody, SPG 13 Antibody

Size :

200 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

LK1

Immunogen :

Recombinant human HSP60

Immunogen Species :

Human

Accession Number :

NP_002147.2

Swiss-Prot :

P10809

Applications :

WB IHC IP ELISA FCM

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt Bv Dg Ck Dr GP Hm Mk Pg Rb Sh Xe Pl Sw

Species Reactivity Full Name :

Human Mouse Rat Bovine Dog Chicken Fruit Fly (Drosophila melanogaster) Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit Sheep African clawed frog (Xenopus laevis) Plant Soybean Silkworm (Bombyx mori)

Antibody Dilution :

WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~60kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-110B, RRID: AB_2121277

Certificate of Analysis :

0.05 µg/ml of SMC-110 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Cellular Localization :

Mitochondrion Mitochondrion Matrix

Scientific Background :

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).

References :

1. Hartl, F.U. (1996) Nature 381: 571-579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366. 3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858. 4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. 10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556. 11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-110_Hsp60_Antibody_LK-1_ICC-IF_Human_HaCaT-cells_1.png SMC-110_Hsp60_Antibody_LK-1_IHC_Human_colon-carcinoma_40x_1.png SMC-110_Hsp60_Antibody_LK-1_WB_Human_Cell-line-lysates_1.png SMC-110_Hsp60_Antibody_LK-1_IHC_Mouse_backskin_1.png SMC-110_Hsp60_Antibody_LK1_ICC-IF_Human_skin-Fibroblasts_1.png

more info or order :

StressMarq Biosciences product webpage