ELISA/assay

Cystatin C EIA Kit

SKT-219-96

96 well

432.00 USD

Cystatin C EIA Kit

Colorimetric measurement of human cystatin C levels

Cystatin C

432.00 USD

USD

Cell Signaling Neuroscience Neurodegeneration Alzheimer's Disease Cancer

Cystatin 3 EIA Kit, gamma trace EIA Kit, post-gamma-globulin or neuroendocrine basic polypeptide EIA Kit

96 well

Assay Kits

EIA Kits

StressXpress®

Colorimetric Assay

Sandwich EIA (Enzyme Immunoassay)

Sandwich

Microplate

EIA kit used to measure cystatin C levels in samples.

The Cystatin C EIA kit is designed to quantitatively measure human Cystatin C present in biological samples and tissue culture media. A human Cystatin C standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the Cystatin C present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated Cystatin C monoclonal antibody is added. The plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound Cystatin C Antibody Conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Cystatin C in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

SKC-219A SKC-219B SKC-219C SKC-219D SKC-219E SKC-219F SKC-219G SKC-219H

Clear 96 well Plate Cystatin C Standard StressXpress® Cystatin C Conjugate Assay Buffer Concentrate Wash Buffer Concentrate TMB Substrate Stop Solution Plate Sealer

1 Plate 60 µl 5 ml 28 ml 30 ml 5 ml 5 ml 1 Each

Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cystatin C concentrations were: Sample 1- 7.72 ng/mL, 9.1% CV Sample 2- 5.08 ng/mL, 10.3% CV Sample 3- 4.42 ng/mL, 7.6% CV Sample 4- 0.88 ng/mL, 10.3% CV Inter Assay Precision: Four human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by three operators. The mean and precision of the calculated Cystatin C concentrations were: Sample 1- 7.79 ng/mL, 8.4% CV Sample 2- 5.48 ng/mL, 10.2% CV Sample 3- 4.97 ng/mL, 11.1% CV Sample 4- 1.04 ng/mL, 12.40% CV

Human

Hu

Serum EDTA Plasma Heparin Plasma Urine Tissue Culture Media

40 samples in duplicate

2 Hours

0.058 ng/ml

0.156 - 10 ng/ml

4ºC

Blue Ice

Cystatin C EIA Kit (StressMarq Biosciences, Canada, Cat # SKT-219-96)

Cystatin C is a non-glycosylated protein of low molecular weight (13kDa) in the cystatin superfamily. Cystatin C is produced at a constant rate in all nucleated cells, secreted from cells and thus found in detectable amounts in most body fluids (1,2). Cystatin C belongs to the cysteine proteinase inhibitor group and is associated with several pathological conditions. Imbalance between Cystatin C and cysteine proteinases is associated with diseases such as inflammation, renal failure, cancer, Alzheimer's disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy (1,3,4). Increased levels have been found in autoimmune diseases, with colorectal tumors and metastases, patients with inflammation and in patients on dialysis (5,6,7). Cystatin C is removed from blood plasma by glomerular filtration in the kidneys. It is reabsorbed by the proximal tubular cells and degraded. There is a linear relationship between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C is suggested to be a better marker for GFR than the ubiquitous serum creatinine marker as its serum concentration is not affected by other factors such as age, gender and body mass and Cystatin C has higher sensitivity to detect a reduced GFR than creatinine determination (2,5). Low levels of Cystatin C are found with the breakdown of the elastic laminae and atherosclerosis and abdominal aortic aneurysm (8). There is evident association of Cystatin C levels with the incidence of myocardial infarction, coronary death and angina pectoris, presenting a risk factor for secondary cardiovascular events (9).

1. Pergande M. and Jung K. Clin Chem. 1993 39(9):1885-90. 2. Newman D, et al. Kidney Int'l. 1995 Jan;47(1):312-8. 3. Levy E. Expert Rev. Neurotherapeutics 2008 May;8(5):687-9. 4. Nakashima I, et al. Ann Neurol. 2007 Aug;62(2):197-200. 5. Uchida K. and Gotoh, A. Clin Chim Acta. 2002 Sep;323:121-8. 6. Pucci L, et al. Clin Chem. 2007 Mar;53(3):480-8. 7. Stabuc B, et al. Clin Chem. 2000 Feb;46(2):193-7. 8. Shlipak M, et al. NEJM 2005 May;352(20):2049-60. 9. Koenig W, et al. Clin Chem. 2005 Feb;51(2):321-7.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Kit Booklet MSDS

SKT-219_Cystatin_C_EIA_Kit_Linearity_Recovery_Graph_Fig2.png
SKT-219_Cystatin_C_EIA_Kit_Standard_Cur
ve_Fig1.png

Graph of the Typical Standard Curve for the Cystatin C EIA Kit StressXpress® - SKT-219 Graph of the Linearity Recovery for the Cystatin C EIA Kit StressXpress® - SKT-219