ELISA/assay

Catalase Activity Kit

SKT-215-192

2 x 96 well

393.00 USD

Catalase Activity Kit

Quantitative colorimetric measurement of catalase activity

Catalase

393.00 USD

USD

Cancer Oxidative Stress Cancer Apoptosis

Cas1 Activity Kit, CAT Activity Kit, Cs1 Activity Kit, MGC138422 Activity Kit, MGC138424 Activity Kit

2 x 96 well

Assay Kits

Activity Kits

StressXpress®

Colorimetric Assay

Direct Enzyme Activity Assay

Enzyme Activity

Microplate

Colorimetric assay used to quantitatively measure catalase activity in a variety of samples.

The Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Colorimetric Detection Reagent is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a pink-colored product. The colored product is read at 560 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in pink product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL.

SKC-215A SKC-215B SKC-215C SKC-215D SKC-215E SKC-215F

Clear 96 well Half Area Plates Catalase Standard Assay Buffer Concentrate Hydrogen Peroxide Reagent Colorimetric Detection Reagent Horseradish Peroxidase Concentrate

2 Plates 90 µl 25 ml 5 ml 5 ml 120 µl

Intra Assay Precision: Three human serum samples diluted in Assay Buffer were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were: Sample 1- 1.71 U/mL, 3.5% CV Sample 2- 0.84 U/mL, 4.0% CV Sample 3- 0.48 U/mL, 4.8% CV Inter Assay Precision: Three human serum samples diluted in Assay Buffer were run in duplicates in twenty-one assays run over multiple days by three operators. The mean and precision of the calculated concentrations were: Sample 1- 1.79 U/mL, 11.9% CV Sample 2- 0.94 U/mL, 9.8% CV Sample 3- 0.53 U/mL, 12.3% CV

Species Independent

ALL

Serum EDTA Plasma Heparin Plasma Cell Lysates Tissue Erythrocytes

89 samples in duplicate

45 Minutes

0.052 U/ml

0.156 - 5 U/ml

4ºC

Blue Ice

Catalase Activity Kit (StressMarq Biosciences, Canada, Cat # SKT-215-192)

Hydrogen peroxide, H2O2 is one of the most frequently occurring reactive oxygen species. It is formed either in the environment or as a by-product of aerobic metabolism, superoxide formation and dismutation, or as a product of oxidase activity. Both excessive hydrogen peroxide and its decomposition product hydroxyl radical, formed in a Fenton-type reaction, are harmful for most cell components. Its rapid removal is essential for all aerobically living prokaryotic and eukaryotic cells (1,2). Hydrogen peroxide however can act as a second messenger in signal transduction pathways, in immune cell activation, inflammation processes, cell proliferation, and apoptosis (3-5). One of the most efficient ways of removing peroxide is through the enzyme catalase, which is encoded by a single gene, and is highly conserved among species (6-8). Mammals, including humans and mice, express catalase in all tissues, and a high concentration of catalase can be found in the liver, kidneys and erythrocytes (9,10). The expression is regulated at transcription, post-transcription and post-translation levels6, (11). High catalase activity is detected in peroxisomes (12). More recently, short wavelength UV radiation has been shown to produce reactive oxygen species (ROS) through the action of catalase (13). This response is thought to act as a mechanism to protect DNA by converting damaging UV radiation into ROS species that can be metabolized and detoxified by cellular antioxidant enzymes.

1. Cabiscol E, Tamarit J, Ros J. (2000) Int. Microbiol. 3:3–8. 2. Kirkman HN, Gaetani GF. (2007) Trends Biochem Sci. 32:44–50. 3. Peus D, et al. Free Radic. (1999) Biol. Med. 27:1197–1202. 4. Rahman I, and Adcock IM. (2006) Eur. Respir. J. 28:219–242. 5. Veal EA, Day AM, Morgan BA.(2007) Mol. Cell. 26:1–14. 6. Reimer, DL, Bailley, J, Singh, SM. (1994) Genomics. 21:325–336. 7. Quan, . Korneluk, RG Tropak, MB, Gravel, RA. (1986) Nucleic Acids Res. 14:5321–5335. 8. Nakashima, H., et al. (1989) Gene. 79:279–288. 9. Deisseroth, A, Dounce, AL. (1970) Physiol. Rev. 50:319–375. 10. Schisler, NJ, Singh, SM. (1987) Genome. 29:748–760. 11. Masters, C, Pegg, M, Crane, D. (1986) Mol. Cell Biochem. 70:113–120. 12. Chance, B, Sies, H, Boveris, A. (1979) Physiol. Rev. 59:527–605. 13. Heck, DE, Vetrano, AM, Mariano, TM & Laskin, JD. (2003) J. Biol. Chem. 278:22432–22436.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Kit Booklet MSDS

SKT-215_Catalase_Activity_Kit_Linearity_Recovery_Graph_Fig3.png SKT-215_Catalase_Activity_Kit_Chemical_Equation_of_Catalase_Reaction_Fig1.png
SKT-215_Catalase_Activity_Kit_Standard_
Curve_Fig2.png

Graph of the Typical Standard Curve for the Catalase Activity Kit StressXpress® - SKT-215 Graph of the Linearity Recovery for the Catalase Activity Kit StressXpress® - SKT-215 Chemical Equation of the Catalase Reaction for the Catalase Activity Kit StressXpress® - SKT-215