product summary
Loading...
company name :
StressMarq Biosciences
product type :
ELISA/assay
product name :
Blood Urea Nitrogen (BUN) Detection Kit
catalog :
SKT-213
quantity :
96 well x2
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SKT-213
Product Name :
Blood Urea Nitrogen (BUN) Detection Kit
Description :
Quantitative colorimetric measurement of urea nitrogen
Size :
96 well x2
Research Area(s) :
Cell Signaling Cardiovascular System
Alternative Name(s) :
BUN, Blood urea nitrogen
Product Type :
Detection Kits
Detection Method :
Colorimetric Assay
Assay Type :
Direct Quantitative Assay
Assay Type Abbreviation :
Direct
Utility :
Colorimetric assay used to quantitatively measure urea nitrogen in a variety of samples.
Assay Overview :
The Blood Urea Nitrogen (BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14.
Precision :
Intra-Assay Precision (Within Run Precision) To determine Intra-Assay Precision, three samples of known concentration were assayed at different times, with different operators. The intra-assay coefficient of variation of the BUN within assays was determined to be <10%. Inter-Assay Precision (Between Run Precision) To determine Inter-Assay Precision, three samples of known concentration were assayed at different times, with different operators. The inter-assay coefficient of variation of the BUN between assays was determined to be <20%.
Species Reactivity :
Species Independent
Sample Type(s) :
Serum Plasma Urine Saliva Tissue Culture Media
Number of Samples :
88 samples in duplicate
Incubation Time :
30 Minutes
Sensitivity :
0.042 mg/dl
Assay Range :
0.156 - 10 mg/dl
Scientific Background :
Urea is a nitrogenous waste product formed in the liver during protein metabolism and excreted by the kidneys. Blood Urea Nitrogen (BUN) levels are commonly used to assess renal function, with normal adult values ranging from 7–21 mg/dL. Elevated BUN (azotemia) may indicate kidney dysfunction, dehydration, or systemic illness. In neuroscience, impaired renal clearance of urea and other metabolites can contribute to cognitive dysfunction and neuroinflammation. Uremic toxins have been implicated in the development of encephalopathy and may exacerbate neurodegenerative conditions. Non-invasive sampling of urea in urine or saliva, alongside serum creatinine, provides a valuable tool for monitoring systemic health in neurological research.
Product Citation PMIDS :
40977851 40650665 39296670 38157259 38237547 38024532 37833817 37315436 35865941 35815260 34938344 32269729 33101449 31894319 31176164 31049139 30713579 28396669
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.