ELISA/assay

Alpha Synuclein Oligomer ELISA Kit

SKT-143

96 well

Alpha Synuclein Oligomer ELISA Kit

Colorimetric detection of oligomeric alpha synuclein

96 well

Neuroscience Neurodegeneration Parkinson's Disease Synuclein Multiple System Atrophy

α syn oligomers, aSyn oligomers, prefibrillar α syn aggregates, alpha synuclein, α syn, aSyn, SNCA, Non A4 / Non Aβ Component of AD Amyloid, NACP, PARK1, PARK4

ELISA Kits

Colorimetric Assay

Sandwich ELISA

Sandwich

ELISA kit used to detect/quantitate the presence of alpha synuclein oligomers

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at Room Tempterature for 1 hour with shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at Room Temperature for 1 hour with shaking at 600rpm 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at Room Temperature for 30 minutes with shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well.

Human Mouse

Brain homogenate

40 samples in duplicate

3 hours

10 pg/mL

15.6 – 1,000 pg/mL

Alpha synuclein oligomers represent a soluble, neurotoxic form of the presynaptic protein and are increasingly recognized as early drivers of pathology in neurodegenerative disease. These oligomeric species disrupt synaptic vesicle trafficking and neurotransmission, impairing presynaptic function well before the formation of mature fibrillar aggregates. Localized effects extend to mitochondria, where oligomers induce oxidative stress, lysosomal dysfunction, and endoplasmic reticulum stress, contributing to neuronal vulnerability in Parkinson’s disease (PD) and related synucleinopathies. Their capacity to propagate in a prion like manner and initiate early synaptic and inflammatory disturbances underscores their importance in disease progression and positions them as high value targets for biomarker development and therapeutic intervention. StressMarq’s Alpha Synuclein Oligomer ELISA has demonstrated higher affinity to oligomers than to PFFs or monomers.