Nitrotyrosine ELISA Kit | StressMarq Biosciences SKT-126-96 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

StressMarq Biosciences

product type :

ELISA/assay

product name :

Nitrotyrosine ELISA Kit

catalog :

SKT-126-96

quantity :

96 well

price :

399.00 USD

citations: 1

Reference
Zimnol A, Amann K, Mandel P, Hartmann C, Schupp N. Angiotensin II type 1a receptor-deficient mice develop angiotensin II-induced oxidative stress and DNA damage without blood pressure increase. Am J Physiol Renal Physiol. 2017;:ajprenal.00183.2017 pubmed publisher

product information

Catalog No :

SKT-126-96

Product Name :

Nitrotyrosine ELISA Kit

Description :

Colorimetric detection of Nitrotyrosine

Target :

Nitrotyrosine

2021 List Price :

399.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Cell Signaling Post-translational Modifications Nitration Neuroscience Neurodegeneration Alzheimer's Disease Parkinson's Disease

Alternative Name(s) :

Nitro tyrosine ELISA Kit, 3-Nitrotyrosine ELISA Kit, 3-Nitro-L-tyrosine ELISA Kit, 3 Nitrotyrosine ELISA Kit, 3-NT ELISA Kit, 3NT ELISA Kit

Size :

96 well

Category :

Assay Kits

Product Type :

ELISA Kits

Trademark :

MOLECULAR SIGNATURE®

Detection Method :

Colorimetric Assay

Assay Type :

Competitive ELISA (Enzyme-linked Immunosorbent Assay)

Assay Type Abbreviation :

Competitive

Platform :

Microplate

Utility :

ELISA kit used to quantitate Nitrotyrosine in samples.

Assay Overview :

1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.

Component No. :

SKC-126A SKC-126C SKC-126F SKC-0001 SKC-0002 SKC-0003 SKC-0004 SKC-0005 SKC-0009

Item :

Nitrosylated BSA Coated Plate Nitrotyrosine Standard Nitrotyrosine HRP Conjugated Monoclonal Antibody Sample and Standard Diluent (Red) Nitrotyrosine Antibody Diluent (Blue) Wash Buffer Concentrate (10X) TMB Substrate Stop Solution Plate Cover

Quantity / Size :

1 Plate 1 vial/110µl 1 vial/75µl 1 vial/50mL 1 vial/13mL 1 vial/ 50mL 1 vial/13mL 1 vial/13mL 2

Precision :

Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.

Species Reactivity Full Name :

Species Independent

Species Reactivity Abbreviations :

ALL

Sample Type(s) :

Plasma Serum Cell Lysates Urine

Number of Samples :

38 samples in duplicate

Incubation Time :

1 hour

Sensitivity :

50 nM

Assay Range :

62.5 - 8000 nM

Storage Temperature :

4ºC

Shipping Temperature :

Blue Ice

Cite this Product :

Nitrotyrosine ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-126-96)

Scientific Background :

Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Other Resources Name :

Kit Booklet MSDS Calculations Worksheet

Image Filenames :

SKT-126_Nitrotyrosine_ELISA_kit_Competitve_ELISA_Diagram_Fig1.png SKT-126_Nitrotyrosine_ELISA_kit_Preparation_of_the_Nitrotyrosine_Standards_Fig2.png SKT-126_Nitrotyrosine_ELISA_kit_Triplicate_Sample_Plate_Format_Fig3.png
SKT-126_Nitrotyrosine_ELISA_Kit_Standar
d_Curve.png

Image Alternative Text :

Graph of the Typical Standard Curve for the Nitrotyrosine ELISA Kit MOLECULAR SIGNATURE® - SKT-126 Diagram of the Competitive ELISA for the Nitrotyrosine ELISA Kit MOLECULAR SIGNATURE® - SKT-126 Diagram of the Preparation of Nitrotyrosine Standards for the Nitrotyrosine ELISA Kit MOLECULAR SIGNATURE® - SKT-126 Diagram of the Triplicate Sample Plate Format for the Nitrotyrosine ELISA Kit MOLECULAR SIGNATURE® - SKT-126