Alpha B Crystallin ELISA Kit | StressMarq Biosciences SKT-123-96 product information

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product summary

company name :

StressMarq Biosciences

product type :

ELISA/assay

product name :

Alpha B Crystallin ELISA Kit

catalog :

SKT-123-96

quantity :

96 well

price :

657.00 USD

citations: 1

Reference
Baba T, Oshitari T, Yamamoto S. Level of vitreous alpha-B crystallin in eyes with rhegmatogenous retinal detachment. Graefes Arch Clin Exp Ophthalmol. 2015;253:1251-4 pubmed publisher

product information

Catalog No :

SKT-123-96

Product Name :

Alpha B Crystallin ELISA Kit

Description :

Colorimetric detection of alpha B Crystallin

Target :

alpha B Crystallin

2021 List Price :

657.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock

Alternative Name(s) :

AACRYA ELISA Kit, CRYA2 ELISA Kit, CRYAB ELISA Kit, CTPP2 ELISA Kit, HSPB5 ELISA Kit, NY Ren 27 antigen ELISA Kit, alpha crystallin B ELISA Kit

Size :

96 well

Category :

Assay Kits

Product Type :

ELISA Kits

Trademark :

StressXpress®

Detection Method :

Colorimetric Assay

Assay Type :

Sandwich ELISA (Enzyme-linked Immunosorbent Assay)

Assay Type Abbreviation :

Sandwich

Platform :

Microplate

Utility :

ELISA kit used to quantitate alpha B crystallin concentration in samples.

Assay Overview :

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

Component No. :

SKC-123A SKC-123B SKC-123C SKC-123D SKC-123E SKC-123F SKC-123G SKC-123H SKC-123I SKC-123J SKC-123K

Item :

Anti-Alpha B Crystallin Immunoassay Plate 5X Alpha B Crystallin Extraction Reagent Recombinant Alpha B Crystallin Standard Standard and Sample Diluent 10X Wash Buffer Concentrate Anti-Alpha B Crystallin Biotinylated Antibody Concentrate Anti-Alpha B Crystallin Biotinylated Antibody Diluent Streptavidin: HRP Concentrate Streptavidin: HRP Diluent TMB Substrate Stop Solution

Quantity / Size :

1 Plate 1 vial/10 ml 2 vials 1 vial/ 50 ml 1 vial/100 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml

Species Reactivity Full Name :

Human

Species Reactivity Abbreviations :

Sample Type(s) :

Cell Lysates Tissue Serum

Number of Samples :

40 samples in duplicate

Incubation Time :

30 minutes

Sensitivity :

0.009 ng/ml

Assay Range :

0.352 - 22.5 ng/ml

Storage Temperature :

4ºC

Shipping Temperature :

Blue Ice

Cite this Product :

Alpha B Crystallin ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-123-96)

Scientific Background :

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. alpha and beta families are further divided into acidic and basic groups (alpha-A and alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

References :

1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577. 9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Other Resources Name :

Kit Booklet MSDS

Image Filenames :

SKT-123_Alpha_B_Crystallin_ELISA_Kit_St
andard_Curve.png

Image Alternative Text :

Graph of the Typical Standard Curve for the Alpha B Crystallin ELISA Kit StressXpress® - SKT-123