ELISA/assay

DNA Damage (8-OHdG) ELISA Kit

SKT-120

96 well

DNA Damage (8-OHdG) ELISA Kit

Colorimetric detection of 8-hydroxy-2-deoxy Guanosine

96 well

Cancer Oxidative Stress Cell Signaling Post-translational Modifications Oxidation Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Damage and Repair

8 hydroxyguanine, 8-OH-dG, 8-OHdG, 80G, 8OHG, DNA Damage

DNA Damage (8-OHdG) ELISA Kit

Colorimetric Assay

Competitive ELISA (Enzyme-linked Immunosorbent Assay)

Competitive

ELISA Kit for 8-OHdG detection in samples.

1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.

Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.

Species Independent

Urine Cell Lysates Plasma Sample matrices

39 samples in duplicate

1 hour

0.59 ng/mL

0.94 - 60 ng/mL

Cross-reactivity: 8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a widely recognized biomarker of oxidative DNA damage, formed when reactive oxygen and nitrogen species modify guanine bases. This hydroxylation process occurs during normal metabolism and is amplified by environmental stressors that increase oxidative load. Elevated levels of 8-OHdG are strongly associated with aging and a range of chronic conditions, including cancer, diabetes, hypertension, and neurodegenerative diseases. In neuroscience, 8-OHdG serves as a critical indicator of oxidative stress in brain tissue, where neurons are particularly vulnerable due to high metabolic activity and lipid content. Increased 8-OHdG levels have been linked to the progression of Alzheimer’s, Parkinson’s, and other neurodegenerative disorders, making it a valuable tool for monitoring disease onset and therapeutic response. 8-OHdG can exist as a free nucleoside or be incorporated into DNA. In biological samples such as plasma, cell lysates, and tissues, its detection is complex; however, urine provides a more reliable matrix for measuring free 8-OHdG due to efficient renal filtration. Typical urinary concentrations range from 2.7–13 ng/mg creatinine, while plasma levels are significantly lower, often between 4–21 pg/mL as measured by LC-MS. By tracking 8-OHdG levels, researchers gain insight into cellular oxidative damage, enabling early detection and evaluation of antioxidant-based interventions in neurodegenerative disease research.

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