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product summary
company name :
StressMarq Biosciences
product type :
ELISA/assay
product name :
DNA Damage (8-OHdG) ELISA Kit
catalog :
SKT-120-96S
quantity :
96 well
price :
399.00 USD
citations: 45
Reference
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Jha J, Thallas Bonke V, Banal C, Gray S, Chow B, Ramm G, et al. Podocyte-specific Nox4 deletion affords renoprotection in a mouse model of diabetic nephropathy. Diabetologia. 2016;59:379-89 pubmed publisher
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Wu Y, Xue B, Li X, Liu H. Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress. Neural Regen Res. 2012;7:2583-91 pubmed publisher
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product information
Catalog No :
SKT-120-96S
Product Name :
DNA Damage (8-OHdG) ELISA Kit
Description :
Colorimetric detection of 8-hydroxy-2-deoxy Guanosine
Target :
DNA Damage (8-OHdG)
2021 List Price :
399.00 USD
Currency :
USD
Research Area(s) :
Cancer Oxidative Stress Cell Signaling Post-translational Modifications Oxidation Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Damage and Repair
Alternative Name(s) :
8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, 8-OHdG ELISA Kit, DNA Damage ELISA Kit
Size :
96 well
Category :
Assay Kits
Product Type :
ELISA Kits
Trademark :
MOLECULAR SIGNATURE®
Detection Method :
Colorimetric Assay
Assay Type :
Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation :
Competitive
Platform :
Microplate
Utility :
ELISA Kit for 8-OHdG detection in samples.
Assay Overview :
1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
Component No. :
SKC-120A SKC-120C SKC-120F SKC-0001 SKC-0002 SKC-0003 SKC-0004 SKC-0005 SKC-0009
Item :
8-hydroxy-2-deoxy Guanosine : BSA Coated Plate 8-hydroxy-2-deoxy Guanosine Standard 8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody Sample and Standard Diluent 8-hydroxy-2-deoxy Guanosine Antibody Diluent Wash Buffer Concentrate TMB Substrate Stop Solution Plate Cover
Quantity / Size :
1 Plate 1 vial/ 100uL 1 vial/75uL 1 vial/50mL 1 vial/13mL 1 vial/50mL 1 vial/13mL 1 vial/13mL  2 covers
Precision :
Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.
Species Reactivity Full Name :
Species Independent
Species Reactivity Abbreviations :
ALL
Sample Type(s) :
Urine Cell Lysates Plasma Sample matrices
Number of Samples :
39 samples in duplicate
Incubation Time :
1 hour
Sensitivity :
0.59 ng/mL
Assay Range :
0.94 - 60 ng/mL
Specificity :
Cross-reactivity: 8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.
Storage Temperature :
4ºC and -20ºC
Shipping Temperature :
Blue Ice
Cite this Product :
DNA Damage (8-OHdG) ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-120-96S)
Scientific Background :
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
References :
1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120. 2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34. 3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids. 4. Lin H., et al. (2004) Biochem J. 380: 541-548. 5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666. 6. Lee J., et al. (2005) Hypertension 45: 986-990. 7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152. 8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75. 9. Kuo H., et al. (2007) Mutat Res. 631:62-68. 10. Shen J., et al. (2007) Cancer 109: 574-580. 11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636. 12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110. 13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236. 14. Floyd R.A. (1990) FASEB J 4: 2587-2597.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Other Resources Name :
Kit Booklet MSDS Calculations Worksheet
Image Filenames :
SKT-120_DNA_Damage_8-OHdG_ELISA_Kit_Urine_Spike_Assay_Fig3.png SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Preparation_of_the_8-OHdG_Standards_Fig4.png SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Triplicate_Sample_Plate_Format_Fig5.png SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Calculations_Worksheet_Fig7.png
SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Com
petitive_ELISA_Diagram_Fig2.png
SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Standard_Curve_Fig6.png SKT-120_DNA_Damage_8-OHdG_Oxidation_of_Guanosine_Fig1.png
Image Alternative Text :
Graph of the Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120 Chemical Equation of the Oxidation of Guanosine for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120 Diagram of the 8-OHdG Competitive ELISA for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120 Graph of the Urine Spike Assay for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120 Diagram of the Preparation of the 8-OHdG Standards for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120 Diagram of the Triplicate Sample Plate Format for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120 Preview of the Calculations Worksheet for the DNA Damage (8-OHdG) ELISA kit MOLECULAR SIGNATURE® - SKT-120
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.