HSP65 ELISA Kit | StressMarq Biosciences SKT-113-96 product information

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product summary

company name :

StressMarq Biosciences

product type :

ELISA/assay

product name :

HSP65 ELISA Kit

catalog :

SKT-113-96

quantity :

96 well

price :

572.00 USD

product information

Catalog No :

SKT-113-96

Product Name :

HSP65 ELISA Kit

Description :

Colorimetric detection of M. bovis/M. tuberculosum HSP65 in human samples

Target :

HSP65

2021 List Price :

572.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock

Alternative Name(s) :

60kDa chaperonin 2 ELISA Kit, Cell wall protein A ELISA Kit, groEL Protein 2 ELISA Kit, GroEL2 ELISA Kit, M. Tuberculosis cell wall protein A ELISA Kit, M. Tuberculosis HSP65 ELISA Kit

Size :

96 well

Category :

Assay Kits

Product Type :

ELISA Kits

Trademark :

StressXpress®

Detection Method :

Colorimetric Assay

Assay Type :

Sandwich ELISA (Enzyme-linked Immunosorbent Assay)

Assay Type Abbreviation :

Sandwich

Platform :

Microplate

Utility :

ELISA kit used to quantitate M. bovis/M. tuberculosum HSP65 concentration in human samples

Assay Overview :

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

Component No. :

SKC-113A SKC-113B SKC-113C SKC-113D SKC-113E SKC-113F SKC-113G SKC-113H SKC-113I SKC-113J SKC-113K

Item :

Anti-Hsp65 Immunoassay Plate 5X Hsp65 Extraction Reagent Recombinant Hsp65 Standard Standard and Sample Diluent 10X Wash Buffer Concentrate Anti-Hsp65 Biotinylated Antibody Concentrate Anti-Hsp65 Biotinylated Antibody Diluent Streptavidin: HRP Concentrate Streptavidin: HRP Diluent TMB Substrate Stop Solution

Quantity / Size :

1 Plate 1 vial/10 ml 2 vials 1 vial/ 50 ml 1 vial/100 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml

Species Reactivity Full Name :

Mycobacterium Bovis Mycobacterium Tuberculosum

Species Reactivity Abbreviations :

Sample Type(s) :

Cell Lysates Tissue Serum

Number of Samples :

40 samples in duplicate

Incubation Time :

30 minutes

Sensitivity :

0.06 ng/ml

Assay Range :

0.344 - 22 ng/ml

Storage Temperature :

4ºC

Shipping Temperature :

Blue Ice

Cite this Product :

HSP65 ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-113-96)

Scientific Background :

HSP65 is a member of the HSP60 family of heat shock proteins (2, 3). HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6).

References :

1. Koll H., et al. (1992) Cell. 68: 1163-1175. 2. Thole J.E.R., et al. (1985) Infect. Immuno. 50: 800-806. 3. Thole J.E.R., et al., (1987) Infect. Immuno. 55: 1466-1475. 4. Shinnick T.M. Sweetser D., Thole J., van Embden J. and Young R.A. (1987) Infect. Immuno. 55: 1932-1935. 5. Van Eden W., et al. (1988) Nature 331: 171-178. 6. Cobelens P.M., et al. (2002) Rheumatology 41: 775-779.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Other Resources Name :

Kit Booklet MSDS

Image Filenames :

SKT-113_Hsp65_ELISA_Kit_Standard_Curve.png

Image Alternative Text :

Graph of the Typical Standard Curve for the HSP65 ELISA Kit StressXpress® - SKT-113