HSP27 ELISA Kit | StressMarq Biosciences SKT-109-96 product information

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product summary

company name :

StressMarq Biosciences

product type :

ELISA/assay

product name :

HSP27 ELISA Kit

catalog :

SKT-109-96

quantity :

96 well

price :

524.00 USD

citations: 1

Reference
Miyazaki D, Nakamura A, Hineno A, Kobayashi C, Kinoshita T, Yoshida K, et al. Elevation of serum heat-shock protein levels in amyotrophic lateral sclerosis. Neurol Sci. 2016;37:1277-81 pubmed publisher

product information

Catalog No :

SKT-109-96

Product Name :

HSP27 ELISA Kit

Description :

Colorimetric detection of HSP27

Target :

HSP27

2021 List Price :

524.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Cytoskeleton Microfilaments Actin Actin Assembly Cell Signaling Cytoskeleton Microtubules Cancer Tumor Biomarkers Cardiovascular System Atherosclerosis Cardiovascular System Heart Contractility

Alternative Name(s) :

28kDa heat shock protein ELISA Kit, CMT2F ELISA Kit, HSP25 ELISA Kit, HSP27 ELISA Kit, HSP28 ELISA Kit, HSPB1 ELISA Kit, SRP27 ELISA Kit

Size :

96 well

Category :

Assay Kits

Product Type :

ELISA Kits

Trademark :

StressXpress®

Detection Method :

Colorimetric Assay

Assay Type :

Sandwich ELISA (Enzyme-linked Immunosorbent Assay)

Assay Type Abbreviation :

Sandwich

Platform :

Microplate

Utility :

ELISA kit used to quantitate HSP27 concentration in samples.

Assay Overview :

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

Component No. :

SKC-109A SKC-109B SKC-109C SKC-109D SKC-109E SKC-109F SKC-109G SKC-109H SKC-109I SKC-109J SKC-109K

Item :

Anti-Hsp27 Immunoassay Plate 5X Hsp27 Extraction Reagent Recombinant Hsp27 Standard Standard and Sample Diluent 10X Wash Buffer Concentrate Anti-Hsp27 Biotinylated Antibody Concentrate Anti-Hsp27 Biotinylated Antibody Diluent Streptavidin: HRP Concentrate Streptavidin: HRP Diluent TMB Substrate Stop Solution

Quantity / Size :

1 Plate 1 vial/10 ml 2 vials 1 vial/ 50 ml 1 vial/100 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml

Species Reactivity Full Name :

Human

Species Reactivity Abbreviations :

Sample Type(s) :

Serum Plasma Cell Lysates Tissue

Number of Samples :

40 samples in duplicate

Incubation Time :

30 minutes

Sensitivity :

0.04 ng/ml

Assay Range :

0.2 - 13 ng/ml

Storage Temperature :

4ºC

Shipping Temperature :

Blue Ice

Cite this Product :

HSP27 ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-109-96)

Scientific Background :

HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermotolerance invivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). Looking for more information on HSP27? Visit our new HSP27 Scientific Resource Guide at http://www.HSP27.com.

References :

1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19):1558-70. 5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Other Resources Name :

Kit Booklet Lot No. 1102 MSDS

Image Filenames :

SKT-109_Hsp27_ELISA_Kit_Standard_Curve.png

Image Alternative Text :

Graph of the Typical Standard Curve for the HSP27 ELISA Kit StressXpress® - SKT-109