ELISA/assay

HSP90 alpha ELISA Kit

SKT-107-96

96 well

625.00 USD

HSP90 alpha ELISA Kit

Colorimetric detection of HSP90 alpha

HSP90

625.00 USD

USD

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

HSP86 ELISA Kit, HSP89A ELISA Kit, HSP90A ELISA Kit, HSP90AA1 ELISA Kit, HSPC1 ELISA Kit, HSPCA ELISA Kit, HsoCAL3 ELISA Kit, HSP90alpha ELISA

96 well

Assay Kits

ELISA Kits

StressXpress®

Colorimetric Assay

Sandwich ELISA (Enzyme-linked Immunosorbent Assay)

Sandwich

Microplate

ELISA kit used to quantitate HSP90 alpha concentration in samples.

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature, 20-25 °C for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

SKC-107A SKC-107B SKC-107C SKC-107D SKC-107E SKC-107F SKC-107G SKC-107H SKC-107I SKC-107J SKC-107K

Anti-Hsp90a Immunoassay Plate 5X Hsp90a Extraction Reagent Recombinant Hsp90a Standard Standard and Sample Diluent 10X Wash Buffer Concentrate Anti-Hsp90a Biotinylated Antibody Concentrate Anti-Hsp90a Biotinylated Antibody Diluent Streptavidin: HRP Concentrate Streptavidin: HRP Diluent TMB Substrate Stop Solution

1 Plate 1 vial/10 ml 2 vials 1 vial/ 50 ml 1 vial/100 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml

Human Goat Avian (Zebra finch)

Hu Gt Av

Cell Lysates Tissue Serum Whole Blood

40 samples in duplicate

30 minutes

0.117 ng/ml

0.44 - 28 ng/ml

4ºC and -20ºC

Blue Ice

HSP90 alpha ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-107-96)

HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90- regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now known to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(3). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

1. Arlander S.J.H., et al. (2003) J Biol Chem. 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem. 59: 157-186. 3. Neckers L, et al. (2002) Trends Mol Med. 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med. 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev. 18: 306-360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med. 217: 420-434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA. 91: 8324- 8328.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Kit Booklet Lot No. BH585999 Kit Booklet Lot No. SH585170 Kit Booklet Lot No. 1008 MSDS

SKT-107_HSP90_Alpha_ELISA_kit_Typical_S
tandard_Curve_Lot-SH585170.png

Graph of the Typical Standard Curve for the HSP90 Alpha ELISA kit StressXpress® - SKT-107