HSC70 ELISA Kit | StressMarq Biosciences SKT-106-96 product information

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product summary

company name :

StressMarq Biosciences

product type :

ELISA/assay

product name :

HSC70 ELISA Kit

catalog :

SKT-106-96

quantity :

96 well

price :

657.00 USD

citations: 1

Reference
BoÄko A, Vetchinin S, Sapozhnikov A, KOvalenko E. [Alterations in heat shock protein 70 kDa levels in human neutrophils under the heat shock conditions]. Bioorg Khim. 2014;40:528-40 pubmed

product information

Catalog No :

SKT-106-96

Product Name :

HSC70 ELISA Kit

Description :

Colorimetric detection of HSC70

Target :

HSC70

2021 List Price :

657.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock

Alternative Name(s) :

HSC54 ELISA Kit, HSC71 ELISA Kit, HSC73 ELISA Kit, HSP71 ELISA Kit, HSP73 ELISA Kit, HSPA10 ELISA Kit, HSPA8 ELISA Kit, LAP1 ELISA Kit, NIP71 ELISA Kit

Size :

96 well

Category :

Assay Kits

Product Type :

ELISA Kits

Trademark :

StressXpress®

Detection Method :

Colorimetric Assay

Assay Type :

Sandwich ELISA (Enzyme-linked Immunosorbent Assay)

Assay Type Abbreviation :

Sandwich

Platform :

Microplate

Utility :

ELISA kit used to quantitate HSC70 concentration in samples.

Assay Overview :

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

Component No. :

SKC-106A SKC-106B SKC-106C SKC-106D SKC-106E SKC-106F SKC-106G SKC-106H SKC-106I SKC-106J SKC-106K

Item :

Anti-Hsc70 Immunoassay Plate 5X Hsc70 Extraction Reagent Recombinant Hsc70 Standard Standard and Sample Diluent 10X Wash Buffer Concentrate Anti-Hsc70 Biotinylated Antibody Concentrate Anti-Hsc70 Biotinylated Antibody Diluent Streptavidin: HRP Concentrate Streptavidin: HRP Diluent TMB Substrate Stop Solution

Quantity / Size :

1 Plate 1 vial/10 ml 2 vials 1 vial/ 50 ml 1 vial/100 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml

Species Reactivity Full Name :

Human Avian (Zebra finch)

Species Reactivity Abbreviations :

Hu Av

Sample Type(s) :

Cell Lysates Tissue Serum Whole Blood

Number of Samples :

40 samples in duplicate

Incubation Time :

30 minutes

Sensitivity :

1.54 ng/ml

Assay Range :

2.34 - 150 ng/ml

Storage Temperature :

4ºC

Shipping Temperature :

Blue Ice

Cite this Product :

HSC70 ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-106-96)

Scientific Background :

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).

References :

1. Brown C.L., et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty, et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 6. Brown C.L., et al. (1996) J. Biol. Chem. 271(2): 833-840.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Other Resources Name :

Kit Booklet Lot No. 131007 MSDS

Image Filenames :

SKT-106_Hsc70_ELISA_Kit_Standard_Curve.png

Image Alternative Text :

Graph of the Typical Standard Curve for the HSC70 ELISA kit StressXpress® - SKT-106