Alpha Synuclein Pre-formed Fibrils (Type 1)
StressMarq Biosciences
catalog: SPR-322
citations: 17
quantity: 50 µg
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Alpha Synuclein Monomers (Type 1)
StressMarq Biosciences
catalog: SPR-321
citations: 6
quantity: 50 µg
price:
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Alpha Synuclein N-Terminal Acetylated Monomers (Type 1)
StressMarq Biosciences
catalog: SPR-331
citations: 4
quantity: 100 µg
price:
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Alpha Synuclein N-Terminal Acetylated Pre-formed Fibrils (Type 1)
StressMarq Biosciences
catalog: SPR-332
citations: 5
quantity: 100 µg
price:
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Alpha Synuclein Oligomers (Kinetically Stable)
StressMarq Biosciences
catalog: SPR-484
citations: 5
quantity: 100 µg
price:
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Alpha Synuclein A53T Mutant Monomers (Type 1)
StressMarq Biosciences
catalog: SPR-325
citations: 7

SDS-PAGE of ~14 kDa A53T Alpha Synuclein Monomer (SPR-325)

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (SPR-325). A much greater increase in fluorescence is seen when 100 uM monomer (SPR-325) is combined with 10 nM of fibrils (SPR-326) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (SPR-325). A much greater increase in fluorescence is seen when 100 uM monomer (SPR-325) is combined with 10 nM of fibrils (SPR-326) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.
quantity: 100 µg
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Alpha Synuclein Monomers (Type 2)
StressMarq Biosciences
catalog: SPR-316
citations: 2
quantity: 100 µg
price:
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Alpha Synuclein Pre-formed Fibrils (Type 2)
StressMarq Biosciences
catalog: SPR-317
citations: 2
quantity: 100 µg
price:
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Alpha Synuclein Oligomers (Dopamine HCL Stabilized)
StressMarq Biosciences
catalog: SPR-466
citations: 2
quantity: 100 µg
price:
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Alpha Synuclein Pre-formed Fibrils: ATTO 594 (Type 1)
StressMarq Biosciences
catalog: SPR-322-A594
citations: 2
quantity: 50 µg
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Alpha Synuclein A53T Mutant Pre-formed Fibrils (Type 1)
StressMarq Biosciences
catalog: SPR-326
citations: 2

Primary rat hippocampal neurons show lewy body inclusion formation when treated with A53T mutant Alpha Synuclein Protein Pre-formed Fibrils (SPR-326) (B) but not when treated with a media control (A). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Primary Antibody: Rabbit anti-pSer129 Antibody. Fibrils were diluted to 1 ug/uL in neuronal media consisting of B27, Glutamax, penicillin/strip, and neurobasalA and sonicated for 1 hour in a water bath. The sonicated stock was then used to achieve the final concentration of 1 ug/mL in the wells.

TEM of A53T alpha synuclein Pre-formed Fibrils (SPR-326)

TEM of A53T alpha synuclein Pre-formed Fibrils (SPR-326)
quantity: 100 µg
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Alpha Synuclein Filaments
StressMarq Biosciences
catalog: SPR-450
citations: 1
quantity: 100 µg
price:
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Alpha Synuclein Oligomers (Epigallocatechin gallate (EGCG) Stabilized)
StressMarq Biosciences
catalog: SPR-469
citations: 1
quantity: 100 µg
price:
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Alpha Synuclein S87N Mutant Monomers
StressMarq Biosciences
catalog: SPR-499


SDS-PAGE of human alpha synuclein S87N monomer under reducing conditions showing the protein purity. Lane 1: Biorad All Blue Standards (3uL), Lane 2: human alpha synuclein S87N monomer (2ug), lane 3: human alpha synuclein S87N monomer (4ug).
quantity: 100 µg
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Alpha Synuclein S87N Mutant Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-500


TEM of human alpha synuclein S87N fibrils. Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm. Method: Samples were prepared for examination in the transmission electron microscope using the ‘direct application method’ (Doane and Anderson 1987).
quantity: 100 µg
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Alpha Synuclein TNG (A53T, S87N, N103G) Mutant Monomers
StressMarq Biosciences
catalog: SPR-503


SDS-PAGE of purified human alpha synuclein TNG (A53T, S87N, N103G) monomer (SPR-503) on a 12% Bis-Tris Gel. 2ug and 4ug of total protein were loaded into the respective lanes. Electrophoresis was run at 200V for 45 minutes with fixed voltage using 1X MES running buffer.

Fibril formation and seeding activity of human alpha synuclein TNG mutant measured by ThT in vitro. Human TNG mutant monomers (SPR-503) self-aggregate faster than human wild-type monomers. Human TNG mutant pre-formed fibrils (SPR-504) rapidly seed mouse wild-type monomers, but not human wild-type monomers, similar to the seeding pattern observed for mouse wild-type pre-formed fibrils.

Fibril formation and seeding activity of human alpha synuclein TNG mutant measured by ThT in vitro. Human TNG mutant monomers (SPR-503) self-aggregate faster than human wild-type monomers. Human TNG mutant pre-formed fibrils (SPR-504) rapidly seed mouse wild-type monomers, but not human wild-type monomers, similar to the seeding pattern observed for mouse wild-type pre-formed fibrils.
quantity: 100 µg
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Alpha Synuclein TNG (A53T, S87N, N103G) Mutant Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-504


TEM of human alpha synuclein TNG (A53T, S87N, N103G) fibrils (SPR-504). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.

SDS-PAGE of purified human alpha synuclein TNG (A53T, S87N, N103G) fibrils (SPR-504) on a 12% Bis-Tris Gel. 2ug and 4ug of total protein were loaded into the respective lanes. Electrophoresis was run at 200V for 45 minutes with fixed voltage using 1X MES running buffer.

Fibril formation and seeding activity of human alpha synuclein TNG mutant measured by ThT in vitro. Human TNG mutant monomers (SPR-503) self-aggregate faster than human wild-type monomers. Human TNG mutant pre-formed fibrils (SPR-504) rapidly seed mouse wild-type monomers, but not human wild-type monomers, similar to the seeding pattern observed for mouse wild-type pre-formed fibrils.
quantity: 100 µg
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Alpha Synuclein S129A Mutant Monomers
StressMarq Biosciences
catalog: SPR-505


SDS-PAGE of purified human alpha synuclein S129A monomer (SPR-505) on a 12% Bis-Tris Gel. 2ug and 4ug of total protein were loaded into the respective lanes. Electrophoresis was run at 200V for 45 minutes with fixed voltage using 1X MES running buffer.

Seeding activity of human alpha synuclein S129A mutant measured by ThT in vitro. Human alpha synuclein S129A monomers (SPR-505) are rapidly seeded by both wild-type and S129A alpha synuclein pre-formed fibrils (SPR-506) with activity comparable to wild-type monomers.

Seeding activity of human alpha synuclein S129A mutant measured by ThT in vitro. Human alpha synuclein S129A monomers (SPR-505) are rapidly seeded by both wild-type and S129A alpha synuclein pre-formed fibrils (SPR-506) with activity comparable to wild-type monomers.
quantity: 100 µg
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Alpha Synuclein S129A Mutant Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-506


TEM of human alpha synuclein S129A fibrils (SPR-506). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.

SDS-PAGE of purified human alpha synuclein S129A fibrils (SPR-506) on a 12% Bis-Tris Gel. 2ug and 4ug of total protein were loaded into the respective lanes. Electrophoresis was run at 200V for 45 minutes with fixed voltage using 1X MES running buffer.

Seeding activity of human alpha synuclein S129A mutant measured by ThT in vitro. Human alpha synuclein S129A fibrils (SPR-506) will rapidly seed both wild-type and S129A alpha synuclein monomers (SPR-505) with comparable activity to wild-type fibrils.
quantity: 100 µg
price:
to the supplier