Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-329
citations: 12
quantity: 100 µg
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Tau (K18) P301L Mutant Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-330
citations: 7
quantity: 100 µg
price:
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Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils: ATTO 488
StressMarq Biosciences
catalog: SPR-329-A488
citations: 7
quantity: 100 µg
price:
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Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils (Baculovirus/Sf9)
StressMarq Biosciences
catalog: SPR-471
citations: 4

Thioflavin T assay of tau pre-formed fibrils (PFFs) combined with tau monomers. The PFFs seed the aggregation of the monomers
quantity: 100 µg
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Tau-441 (2N4R) Wild-Type Monomers
StressMarq Biosciences
catalog: SPR-479
citations: 4
quantity: 100 µg
price:
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Tau-441 (2N4R) Wild-Type Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-480
citations: 5
quantity: 100 µg
price:
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Tau Truncated Fragment (AA297-391) (dGAE) Monomers
StressMarq Biosciences
catalog: SPR-444
citations: 2

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when truncated tau fragment (AA297-391) (dGAE) monomer is combined with truncated tau fragment (AA297-391) (dGAE) preformed fibrils (Type 1).
quantity: 100 µg
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Tau Truncated Fragment (AA297-391) (dGAE) Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-461
citations: 2
quantity: 100 µg
price:
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Tau (K18) Delta K280 Mutant Monomers
StressMarq Biosciences
catalog: SPR-476
citations: 2
quantity: 100 µg
price:
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Tau-441 (2N4R) P301S Mutant Monomers
StressMarq Biosciences
catalog: SPR-327
citations: 2

SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (SPR-327). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (SPR-327)

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (SPR-327). A greater increase in fluorescence is seen when 50 uM monomer (SPR-327) is combined with 10 nM PFFs (SPR-329 or SPR-330), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (SPR-327). A greater increase in fluorescence is seen when 50 uM monomer (SPR-327) is combined with 10 nM PFFs (SPR-329 or SPR-330), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
quantity: 100 µg
price:
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Tau-352 (fetal 0N3R) Wild-Type Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-491
citations: 3

AFM of Fetal Tau 0N3R fibrils. Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in dH2O, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 10 x 10 µm x-y (left) and 2 x 2 µm x-y (right) both with a z-range of 6 nm. Note: AFM images display significant twisting and curvature not observed under TEM.

Tris-glycine SDS-PAGE (12%) of Fetal Tau 0N3R monomers. MW ladder = Precision Plus Protein All Blue prestained standards.
quantity: 100 µg
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Tau (K18) P301L Mutant Monomers
StressMarq Biosciences
catalog: SPR-328
citations: 1
quantity: 100 µg
price:
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Tau Truncated Fragment (AA297-391) (dGAE C322A) Monomers
StressMarq Biosciences
catalog: SPR-445
citations: 1

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when truncated tau fragment (AA297-391) (dGAE C322A) monomer is combined with truncated tau fragment (AA297-391) (dGAE C322A) preformed fibrils (Type 1).
quantity: 100 µg
price:
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Tau Truncated (AA297-391) (dGAE C322A) Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-462
citations: 1
quantity: 100 µg
price:
to the supplier
Tau-441 (2N4R) P301S Mutant Filaments
StressMarq Biosciences
catalog: SPR-463
citations: 1
quantity: 100 µg
price:
to the supplier
Tau (K18) Delta K280 Mutant Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-477
citations: 1
quantity: 100 µg
price:
to the supplier
Tau-441 (2N4R) P301S Mutant Monomers (Baculovirus/Sf9)
StressMarq Biosciences
catalog: SPR-473
citations: 1

Thioflavin T assay of tau pre-formed fibrils (PFFs) combined with tau monomers. The PFFs seed the aggregation of the monomers
quantity: 100 µg
price:
to the supplier
Tau-352 (fetal 0N3R) Wild-Type Monomers
StressMarq Biosciences
catalog: SPR-490
citations: 1

Fibril formation activity of Fetal Tau 0N3R monomers in ThT seeding assay. Monomers readily form fibrils upon the addition of heparin or when seeded by 0N3R pre-formed fibrils. DTT was added to each well to control disulfide bonding during fibril formation.

Fibril formation activity of Fetal Tau 0N3R monomers in ThT seeding assay. Monomers readily form fibrils upon the addition of heparin or when seeded by 0N3R pre-formed fibrils. DTT was added to each well to control disulfide bonding during fibril formation.
quantity: 100 µg
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Tau-441 (2N4R) Wild-Type Monomers (Baculovirus/Sf9)
StressMarq Biosciences
catalog: SPR-496


SDS-PAGE of purified Sf9/Baculovirus-expressed hTau wild-type 2N4R on a 12% Tris-Glycine Gel. Lane 1: Biorad All Blue Standards (3uL). Lane 2: Baculovirus expressed hTau 2N4R Wild-Type (2ug). Lane 3: Lane 2: Baculovirus expressed hTau 2N4R Wild-Type (4ug).

Dot Blot of purified hTau wild-type 2N4R using phospho-specific Tau antibodies comparing E.coli-expressed material to Sf9/Baculovirus-expressed material. Protein was blotted on nitrocellulose, incubated with 1:1000 primary antibody and/or 1:4000 secondary antibodies. Exposed 0.6s second.

Modified/Total phosphorylation PTM spectrum counts reveal up to 20 phosphorylation sites on human wild-type Tau 2N4R monomers expressed using Baculovirus/Sf9 as determined by mass spectrometry. Protein sequence coverage was 76%. Localization probability cutoff set at ≥80% (yellow) or ≥95% (green).
quantity: 100 µg
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Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)
StressMarq Biosciences
catalog: SPR-498


TEM of Tau-441 (2N4R) Wild-Type Pre-Formed Fibrils (Baculovirus/Sf9). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 500 nm.

SDS-PAGE of fibrilized Sf9/Baculovirus-expressed hTau wild-type 2N4R on a 12% Tris-Glycine Gel. Lane 1: Biorad All Blue Standards (3uL). Lane 2: Baculovirus expressed hTau 2N4R Wild-Type PFFs (2ug). Lane 3: Baculovirus expressed hTau 2N4R Wild-Type PFFs (4ug).

Modified/Total phosphorylation PTM spectrum counts reveal up to 20 phosphorylation sites on human wild-type Tau 2N4R monomers expressed using Baculovirus/Sf9 as determined by mass spectrometry. Protein sequence coverage was 76%. Localization probability cutoff set at ≥80% (yellow) or ≥95% (green).
quantity: 100 µg
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Tau dGAE (297-391) Monomers
StressMarq Biosciences
catalog: SPR-501


SDS-PAGE of purified E. coli expressed hTau (dGAE) (SPR-501) on a 12% Tris-Glycine gel.

TEM of Tau dGAE AD-mimic fibrils (SPR-502) generated from Tau dGAE monomers (SPR-501) by shaking 200 rpm at 37oC for 48 hours in 10 mM PB 10 mM DTT pH 7.4 with 200 mM MgCl2 added (Lovestam et al. 2022, eLife). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.

TEM of Tau dGAE AD-mimic fibrils (SPR-502) generated from Tau dGAE monomers (SPR-501) by shaking 200 rpm at 37oC for 48 hours in 10 mM PB 10 mM DTT pH 7.4 with 200 mM MgCl2 added (Lovestam et al. 2022, eLife). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.
quantity: 100 µg
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Tau dGAE (297-391) AD-mimic Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-502


TEM of Tau dGAE AD-mimic fibrils (SPR-502) generated from Tau dGAE monomers (SPR-501) by shaking 200 rpm at 37oC for 48 hours in 10 mM PB 10 mM DTT pH 7.4 with 200 mM MgCl2 added (Lovestam et al. 2022, eLife). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.

SDS-PAGE of Tau dGAE AD-mimic fibrils (SPR-502) on a 12% Tris-Glycine Gel.

TEM of Tau dGAE AD-mimic fibrils (SPR-502). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 500 nm.
quantity: 100 µg
price:
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