Amyloid Beta 1-42 Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-487
citations: 4

TEM of amyloid beta 1-42 fibrils (SPR-487). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 100 nm.

AFM of amyloid beta 1-42 fibrils (SPR-487). Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in dH2O, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 2.5 x 2.5 µm x-y with a z-range of 10 nm.

Western blot of amyloid beta 1-42 monomers (SPR-485, left), oligomers (SPR-488, middle) and fibrils (SPR-487, right) using anti-amyloid beta 6E10 antibody. Amyloid beta constructs at 160 pmol were run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Oligomers observed under TEM/AFM show distinct dimer/trimer bands as well as a signal from ~37-75 kDa (middle). Fibrils observed under TEM/AFM show a signal greater than 100 kDa and a distinct signal in the stacking gel (right).
quantity: 100 µg
price:
to the supplier
Amyloid Beta 1-42 Oligomers
StressMarq Biosciences
catalog: SPR-488
citations: 5

TEM of amyloid beta 1-42 oligomers (SPR-488). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 100 nm.

AFM of amyloid beta 1-42 oligomers (SPR-488). Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in dH2O, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 2.5 x 2.5 µm x-y with a z-range of 10 nm.

Western blot of amyloid beta 1-42 monomers (SPR-485, left), oligomers (SPR-488, middle) and fibrils (SPR-487, right) using anti-amyloid beta 6E10 antibody. Amyloid beta constructs at 160 pmol were run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Oligomers observed under TEM/AFM show distinct dimer/trimer bands as well as a signal from ~37-75 kDa (middle). Fibrils observed under TEM/AFM show a signal greater than 100 kDa and a distinct signal in the stacking gel (right).
quantity: 100 µg
price:
to the supplier
Amyloid Beta Peptide 1-42 Monomers
StressMarq Biosciences
catalog: SPR-485
citations: 2

TEM of amyloid beta 1-42 monomers (SPR-485). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 100 nm.

Western blot of amyloid beta 1-42 monomers (SPR-485, left), oligomers (SPR-488, middle) and fibrils (SPR-487, right) using anti-amyloid beta 6E10 antibody. Amyloid beta constructs at 160 pmol were run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Oligomers observed under TEM/AFM show distinct dimer/trimer bands as well as a signal from ~37-75 kDa (middle). Fibrils observed under TEM/AFM show a signal greater than 100 kDa and a distinct signal in the stacking gel (right).
quantity: 100 µg
price:
to the supplier
Amyloid Beta Pyroglutamate 3-42 Pre-formed Fibrils
StressMarq Biosciences
catalog: SPR-492
citations: 2

AFM of amyloid beta pyroglutamate 3-42 fibrils (catalog# SPR-492). Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in 2% DMSO + 10 mM HCl, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 10 x 10 µm x-y (left) and 5 x 5 µm x-y (middle) and 2 x 2 µm x-y (right), all with a z-range of 6 nm.

Western blot of amyloid beta pyroglutamate 3-42 fibrils (catalog# SPR-492) using anti-amyloid beta 6E10 antibody. Amyloid beta pyroglutamate 3-42 at 160 pmol was run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Compared to monomers re-suspended in 2% DMSO and immediately run on SDS-PAGE, fibrils show monomer depletion, a signal from 37 kDa upwards and a distinct signal in the stacking gel. MW ladder = Precision Plus Dual Xtra prestained standards.
quantity: 100 µg
price:
to the supplier
Amyloid Beta Peptide 1-40 Monomers
StressMarq Biosciences
catalog: SPR-530


SDS-PAGE analysis of Human Synthetic Amyloid Beta Peptide 1-40 Monomers on a 4-12% Bis-Tris gel (left). Western Blot of Human Synthetic Amyloid Beta Peptide 1-40 Monomers using an anti-amyloid beta 1-16 monoclonal antibody [6E10] (Biolegend Cat # 803012) at 1:1000. Secondary antibody was a goat anti-mouse:HRP at 1:4000. Exposed 5 seconds (right).
quantity: 100 µg
price:
to the supplier
Amyloid Beta Peptide 1-40 + C-term Cysteine Monomers
StressMarq Biosciences
catalog: SPR-533


SDS-PAGE analysis of Human Synthetic Amyloid Beta 1-40 C-Term Cysteine Monomers on a 4-12% Bis-Tris gel (left). Western Blot of Human Synthetic Amyloid Beta Peptide 1-40 C-Term Cysteine Monomers using an anti-amyloid beta 1-16 monoclonal antibody [6E10] (Biolegend Cat # 803012) at 1:1000. Secondary antibody was a goat anti-mouse:HRP at 1:4000. Exposed 1 second (right).
quantity: 100 µg
price:
to the supplier