mouse monoclonal (3G11)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 3G11 (SMC-542). Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody (SMC-542) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (4A3)
reactivity: human, rat
application: western blot, immunohistochemistry
reactivity: human, rat
application: western blot, immunohistochemistry
Immunohistochemistry analysis using Mouse Anti-c-Fos Monoclonal Antibody, Clone 4A3 (SMC-543). Tissue: Kidney. Species: Rat. Primary Antibody: Mouse Anti-c-Fos Monoclonal Antibody (SMC-543) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (5B10)
reactivity: human, rat
application: western blot, immunohistochemistry
reactivity: human, rat
application: western blot, immunohistochemistry
Immunohistochemistry analysis using Mouse Anti-c-Fos Monoclonal Antibody, Clone 5B10 (SMC-544). Tissue: Brain. Species: Rat. Primary Antibody: Mouse Anti-c-Fos Monoclonal Antibody (SMC-544) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 10X.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (5B11)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 5B11 (SMC-545). Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody (SMC-545) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (12G11)
reactivity: human, mouse
application: western blot, immunohistochemistry
reactivity: human, mouse
application: western blot, immunohistochemistry
Immunohistochemistry analysis using Mouse Anti-c-Fos Monoclonal Antibody, Clone 4A3 (SMC-546). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-c-Fos Monoclonal Antibody (SMC-546) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.
quantity: 100 µg
price:
to the supplier